Specificity and sensitivity of enzyme-linked immunosorbent assay with five recombinant surface protein antigens of Coxiella burnetii
WANG Peng-cheng1,2, XIONG Xiao-lu1, JIAO Jun1, GONG Wen-ping1, YANG Xiao-mei1, WEN Bo-hai1
1.State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences, Beijing 100071, China; 2.Department of Clinical Laboratory, the 105th Hospital of PLA, Hefei 230031, China
Abstract:In order to evaluate the usefulness of recombinant proteins of Coxiella burnetti as serodiagnostic markers for Q fever, the enzyme-linked immunosorbent assay (ELISA) coated with each of five recombinant surface protein antigens (Com1, GroEL, Mip, OmpH, or RplL) of Coxiella burnetii was assessed for specificity and sensitivity in detection of specific IgG antibodies in sera from mice experimentally infected with C. burnetii and patients suffered from Q fever. As a result, all of 8 sera from C. burnetii-infected mice positively reacted with the five recombinant proteins, except for one serum which negatively reacted with RplL, while all of 8 sera from mice infected with Rickettsia mooseri, Rickettsia heilongjiangensis, or Orientia tsutsugamshi negatively reacted with the five proteins, except for one serum which positively reacted with GroEL. In addition, the average positive ratio of 11 sera from patients with endemic typhus, 13 sera from patients with spotted fever, and 12 sera from patients with tsutsugamushi disease were 22.2% for reaction to Com1, 25.0% for GroEL, 25.0% for Mip, 25.0% for OmpH, and 13.9% for RplL, respectively. Results suggest that the ELISA with Com1, GroEL, Mip, OmpH, or RplL has high specificity and sensitivity for serological diagnosis of Q fever.
汪鹏程, 熊小路, 焦俊, 龚文平, 杨小梅, 温博海. 贝氏柯克斯体5种重组表面蛋白抗原包被酶联免疫吸附试验的特异性和敏感性分析[J]. 中国人兽共患病学报, 2015, 31(12): 1111-1115.
WANG Peng-cheng, XIONG Xiao-lu, JIAO Jun, GONG Wen-ping, YANG Xiao-mei, WEN Bo-hai. Specificity and sensitivity of enzyme-linked immunosorbent assay with five recombinant surface protein antigens of Coxiella burnetii. Chinese Journal of Zoonoses, 2015, 31(12): 1111-1115.
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