Molecular epidemiological characteristics of Listeria monocytogenes isolated from raw meat samples in some regions of Beijing, China
MA Ai-jing1, WANG Yan1, WANG Yi1, LI Dong-xun2, XU Hua-qing3, YUAN Xue-jiao1, LIU Kai1, YE Chang-yun1
1.State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention, Beijing 102206, China; 2.Department of Microbiology, Medical College of Guiyang, Guiyang 550004, China; 3.Department of Dermatology, the Sixth People’ s Hospital of Zhengzhou, Zhengzhou 450000, China
Abstract:Listeria monocytogenes (L. monocytogenes) is one of the most significant human pathogenic bacteria, as it causes serious invasive illnesses and is mainly transmitted through the food chain. Thus, an epidemiologic study, which investigates the prevalence and molecular characteristics of this pathogen involving raw materials contamination, is extremely required to ensure food safety, evaluate the potential health risk, and provide the control strategies. A total of 340 raw meat samples, including pork, beef, lamb, chicken and duck products, were collected from several local markets in Beijing, and the NMKL No. 136 method was selected for the isolation of L. monocytogenes from various raw meat samples. Then, the serological classification, pulsed-field gel electrophoresis (PFGE) analysis and multi-locus sequence typing (MLST) were performed to provide the valuable strain discrimination. Among 21 strains of L. monocytogenes isolated, accounting for 6.2% (21/340) contamination rate, three serotypes were detected and distinguished, including serovars 1/2a (7), 1/2b (4) and 1/2c (10), and the serotype 1/2c accounted for 47.6% of all isolates. The PFGE typing by AscI divided the 21 isolates into 12 pulse types (PTs) that were assigned to 3 clusters. The result of MLST showed 7 different sequence types and the predominant STs was ST9 (10). Accordingly, the food sources contaminated by L. monocytogenes was confirmed in Beijing by our study, and serotype 1/2c, GX6A16.CN0004 and ST9 were the predominant serotype, pulse type and sequence type, respectively. The result of molecular subtypes indicated that common serotypes, PTs and STs in Beijing had the similar prevalent trends as that in rest of China.
马爱静, 王艳, 王毅, 李东迅, 许华青, 袁雪娇, 刘凯, 叶长芸. 北京市一些地区生肉标本中单增李斯特菌的分离及其分子流行病学特征分析[J]. 中国人兽共患病学报, 2015, 31(5): 403-407.
MA Ai-jing, WANG Yan, WANG Yi, LI Dong-xun, XU Hua-qing, YUAN Xue-jiao, LIU Kai, YE Chang-yun. Molecular epidemiological characteristics of Listeria monocytogenes isolated from raw meat samples in some regions of Beijing, China. Chinese Journal of Zoonoses, 2015, 31(5): 403-407.
[1] Vazquez-Boland JA, Kuhn M, Berche P, et al. Listeria pathogenesis and molecular virulence determinants[J]. ClinMicrobiol Rev, 2001, 14(3): 584-640. doi:10.1128/CMR.14.3.584-640.2001 [2] Schlech WF 3rd. Foodborne listeriosis[J]. Clin Infect Dis, 2000, 31(3): 770-775. doi:10.1086/314008 [3] Poulsen KP, Czuprynski CJ. Pathogenesis of listeriosis during pregnancy[J]. Anim Health Res Rev, 2013, 14(1): 30-39. doi:10.1017/S1466252312000242 [4] Bodro M, Paterso DL. Listeriosis in patients receiving biologic therapies[J]. Eur J ClinMicrobiol Infect Dis, 2013, 32(9): 1225-1230. doi:10.1007/s10096-013-1873-1 [5] Schlech WF 3rd, Lavigne PM, Bortolussi RA, et al. Epidemic listeriosis evidence for transmission by food[J]. N Engl J Med, 1983, 308(4): 203-206. doi:10.1056/NEJM198301273080407 [6] Evans JR, Allen AC, Stinson DA, et al. Perinatal listeriosis: report of an outbreak[J]. Pediatr Infect Dis, 1985, 4(3): 237-241. [7] Fleming DW, Cochi SL, MacDonald KL, et al. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis[J]. N Engl J Med, 1985, 312(7): 404-407. doi:10.1056/NEJM198502143120704 [8] Centers for Disease C(2008), Prevention. Outbreak of Listeria monocytogenes infections associated with pasteurized milk from a local dairy Massachusetts[J]. MMWR Morb Mortal Wkly Rep, 2008, 57: 1097-1100. [9] Linnan MJ, Mascola L, Lou XD, et al. Epidemic listeriosis associated with Mexican-style cheese[J]. N Engl J Med, 1988, 319(13): 823-828. doi:10.1056/NEJM198809293191303 [10] Dalton CB, Austin CC, Sobel J, et al. An outbreak of gastroenteritis and fever due to Listeria monocytogenes in milk[J]. N Engl J Med, 1997, 336(2): 100-105. doi:10.1056/NEJM199701093360204 [11] Gottlieb SL, Newbern EC, Griffin PM, et al. Multistate outbreak of Listeriosis linked to turkey deli meat and subsequent changes in US regulatory policy[J]. Clin Infect Dis, 2006, 42(1): 29-36. doi:10.1086/498113 [12] Centers for Disease C, Prevention (2011). Multistate outbreak of listeriosis associated with Jensen Farms cantaloupe United States, August-September 2011[J]. MMWR Morb Mortal Wkly Rep, 2011, 60(39): 1357-1358. [13] Gilmour MW, Graham M, Van Domselaar G, et al. High-throughput genome sequencing of two Listeria monocytogenes clinical isolates during a large foodborne outbreak[J]. BMC Genomics, 2010, 11: 120. doi:10.1186/1471-2164-11-120 [14] Goulet V, Rocourt J, Rebiere I, et al. Listeriosis outbreak associated with the consumption of rillettes in France in 1993[J]. J Infect Dis, 1998, 177(1): 155-160. [15] Bula CJ, Bille J, Glauser MP. An epidemic of food-borne listeriosis in western Switzerland: description of 57 cases involving adults[J]. Clin Infect Dis, 1995, 20: 66-72. [16] Ericsson H, Eklow A, Danielsson-Tham ML, et al. An outbreak of listeriosis suspected to have been caused by rainbow trout[J]. J ClinMicrobiol, 1997, 35(11): 2904-2907. [17] Aureli P, Fiorucci GC, Caroli D, et al. An outbreak of febrile gastroenteritis associated with corn contaminated by Listeria monocytogenes [J]. N Engl J Med, 2000, 342(17): 1236-1241. doi:10.1056/NEJM200004273421702 [18] Lyytikainen O, Autio T, Maijala R, et al. An outbreak of Listeria monocytogenes serotype 3a infections from butter in Finland[J]. J Infect Dis, 2000, 181(5): 1838-1841. doi:10.1086/315453 [19] Chui HX, Li WJ, Zhang XL, et al. Analysis of serotypes and drug resistance of food-borne Listeria monocytogenes in Henan in 2012[J]. Chin J Health Lab Technol, 2014, 12: 1800-1803. (in Chinese) 炊慧霞,李文杰,张秀丽,等.2012年河南省食源性单核细胞增生李斯特菌血清分型及耐药性分析[J].中国卫生检验杂志,2014,12:1800-1803. [20] Hoelzer K, Pouillot R, Dennis S. Listeria monocytogenes growth dynamics on produce: a review of the available data for predictive modeling[J]. Foodborne Pathog Dis, 2012, 9: 661-673. [21] Viswanath P, Murugesan L, Knabel SJ, et al. Incidence of Listeria monocytogenes and Listeria spp. in a small-scale mushroom production facility[J]. Foodborne Pathog Dis, 2013, 9(7): 661-673. doi:10.1089/fpd.2011.1087 [22] Ryua J, Parka SH, Yeoma YS, et al. Simultaneous detection of Listeria species isolated from meat processed foods using multiplex PCR[J]. Food Ctrl, 2013, 32: 659-664. [23] Ragon M, Wirth T, Hollandt F, et al. A new perspective on Listeria monocytogenes evolution[J]. PLoS Pathog, 4(9): e1000146. doi:10.1371/journal. ppat. 1000146 [24] Wang Y, Zhao A, Zhu R, et al. Genetic diversity and molecular typing of Listeria monocytogenes in China[J]. BMC Microbiol, 2012, 12: 119. doi:10.1186/1471-2180-12-119 [25] Cui JH, Li D, Wang LP, et al. Analysis of main pathogens in food between 2004 and 2006 from Xicheng district in Beijing[J]. Chin J Health Lab, 2008, 06: 1163-1165. (in Chinese) 崔京辉, 李达, 王丽萍, 等. 2004-2006年北京市西城区食品中主要致病菌株检测结果及分析[J]. 中国卫生检验杂志,2008,06: 1163-1165. [26] Kiss R, Tirczka T, Szita G, et al. Listeriamonocytogenes food monitoring data and incidence of human listeriosis in Hungary, 2004[J]. Int J Food Microbiol, 112(1): 71-74. doi:10.1016/j.ijfoodmicro. 2006.06.013