Abstract：The aim of this study was to compare three kinds of commercial enzyme-linked immunosorbent assay (ELISA) kits for detection of antibodies against Japanese encephalitis virus (JEV) in swine sera. A total of 90 swine serum samples were tested simultaneously by three kinds of commercial JEV IgG ELISA kits (Keqian, Lvshiyuan and Tianchen), which were used with the most frequently recorded in domestic literatures. Diagnostic test methods were used for evaluatlon. Neutralization test was used as the gold standard’ test. Results showed that all the three kinds of commercial ELISA kits had higher positive detection rate than that of neutralization test (34.4%, 31/90), the rates of Keqian, Lvshiyuan and Tianchen were 50.0% (45/90), 47.8% (43/90) and 38.9% (35/90), respectively. Both of Lvshiyuan and Tianchen had a result of suspicious positive, the rates were 32.2% (29/90) and 4.4% (4/90) respectively. Sensitivity and specificity of Tianchen was 83.87% and 79.66%, respectively, with the highest positive likelihood ratio (4.12); Keqian had the highest sensitivity (90.32%) and a lower specificity (71.19%), with the highest negative likelihood ratio (0.14); while Lvshiyuan had the lowest sensitivity and specificity, which was 41.94% and 16.95%, respectively. There was no significant difference of detected rates between Tianchen and the gold standard’ serum neutralization test with the highest goodness of fit (κ = 0.63). Comparison results of Keqian and Lvshiyuan with neutralization test were significantly different: the κ coefficient was 0.57 and 0.04, respectively. Additionally, internal consistency analysis suggested that all the ELISA kits had good stability. In conclusion, currently commercially available ELISA kits for detection of JEV IgG antibody in swine sera are quite various. A high false negative was still found in these kits compared with neutralization test. Our results suggest the need to improve the insure of authenticity and reliability in use of commercial JEV IgG ELISA kits.
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2015, Vol. 31 
