Cloning and sequence analysis of the partial CO1 gene within mitochondrial DNA of Echinococcus granulosus
YU Jing-feng1, 3, LI Bin1, LIU Xiao-song2, CHANG Jian-hua2, YANG Xiao-ye1, WANG Rui1, YANG Lian-ru1, LI Xiu-xia3, MU Lan3
1.Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture;College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China; 2. Inner Mongolia Academy of Agricultural &Animal Husbandry Sciences,Hohhot 010031, China; 3.School of Basic Medical Science,Inner Mongolia Medical University,Hohhot 010110,China
Abstract:We investigated the inter-specific genetic marker of Echinococcus granulosus, which was used for further study in molecular classification. The genomic DNA of Echinococcus granulosus collected from the infected sheep and people in Inner Mongolia were extracted. The mitochondrial cytochrome oxidase subunit 1 ( CO1) gene was amplified by PCR using universal primers, and then the PCR product was cloned into pGM-T vectors. The insert was sequenced successfully and compared with other cestodes sequences by DNAStar 5.0 and MEGA 4.0. Results showed that the PCR product was 936 bp in length. Compared with other CO1 gene sequences of related cestodes, the homology among Echinococcus granulosus and Echinococcus canadensis were 91.9% and 91.2%. Echinococcus granulosus and Echinococcus canadensis were in the same branch, and bootstrap values (Boostrap) in the highest was 100%. Echinococcus granulosus affiliated branch of the original leaders of the branch belonged to Proteocephalus macrocephalus far apart. It suggested that the CO1 gene sequences were stably conservative and non-host-specific, which can be used as an ideal species among Echinococcus granulosus genetic markers.
于晶峰, 李滨, 刘晓松, 常建华, 杨晓野, 王瑞, 杨莲茹, 李秀霞, 木兰. 细粒棘球蚴线粒体CO1基因的克隆与序列分析[J]. 中国人兽共患病学报, 2015, 31(6): 532-536.
YU Jing-feng, LI Bin, LIU Xiao-song, CHANG Jian-hua, YANG Xiao-ye, WANG Rui, YANG Lian-ru, LI Xiu-xia, MU Lan. Cloning and sequence analysis of the partial CO1 gene within mitochondrial DNA of Echinococcus granulosus. Chinese Journal of Zoonoses, 2015, 31(6): 532-536.
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