Abstract:We aimed to investigate the characteristics of KatG and inhA gene mutations of Mycobacterium tuberculosis (MTB) associated with INH in Guizhou Province, and to evaluate the clinical application value of gene chip in MTB resistance detection. Resistance to INH of 2 738 clinical samples were detected by gene chip method. These samples were determined as MTB positive by PCR-fluorescent probe method. Characteristics of mutations of INH resistance gene KatG and inhA were then analysed. Meanwhile, MTB were isolated from these samples by Lowenstein-Jensen method and INH resistance were detected by ratio method. Susceptibility results determined by these two methods were compared. Of 465 samples in 2 738 MTB nucleic acid positive samples were resistant to INH determined by gene chip method, and the rate was 16.98%. Gene mutations were mainly found in katG 315(AGC→ACC)and the mutation frequency was 78.06%. The inhA -15 (C→T) mutation was in the second place and the katG 315 (AGC→AAC) was in the third place, and the mutation frequency was 16.13% and 5.59%, respectively. A total of 1 493 MTB strains were isolated from the same samples of the above patients. Being determined by conventional ratio method, 255 MTB strains were detected as resistant to INH, with the rate of 17.08%. INH resistance was judged according to the conventional method, and the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of the gene chip method was 85.49%, 97.50%, 87.55%, 97.03% and 95.45%, respectively. In conclusion, gene mutations related with MTB resistance to INH were mainly happened at the KatG 315 (AGC→ACC) and the inhA -15 (C→T) in Guizhou Province. Gene chip is a rapid test method with high sensitivity, specificity and accuracy, which could be used in the fast detection of the MTB drug resistance to INH.
欧维正, 陈峥宏, 陈静, 骆科文, 王燕, 秦万, 蒙俊. 基因芯片技术检测贵州省结核分枝杆菌耐药基因KatG和inhA[J]. 中国人兽共患病学报, 2015, 31(7): 655-658.
OU Wei-zheng, CHEN Zheng-hong, CHEN Jing, LUO Ke-wen, WANG Yan, QIN Wan, MENG Jun. Determination of resistance genes KatG and inhA in Mycobacterium tuberculosis isolates from Guizhou Province by gene chip. Chinese Journal of Zoonoses, 2015, 31(7): 655-658.
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