Abstract：To investigate the potential of mycobacteriophages TM4, Guo1 and D29 in lysing stationary cells of M. tuberculosis. Stationary cells of M. tuberculosis were induced by prolonged closed culture of logarithmic phase M. tuberculosis at 37 ℃,which were proven through drug sensitivity test, auramine o-Nile red fluorescent staining and electron microscope. The most probable number(MPN) method was used to detect whether the stationary cells of M. tuberculosis had been lysed by TM4,Guo1 and D29. Stationary cells of M. tuberculosis were obtained successfully by closed culture for 11m at 37 ℃. The amounts of M. tuberculosis in control, Guo1, TM4,D29, RFP and INH groups by MPN method were respectively 1 100, 23, 23, 600, 673, 887 after different treatments. Compared with control group, the amount of M. tuberculosis in Guo1 and TM4 groups decreased more obviously(P<0.01, P<0.01) than D29 group(P=0.05), compared with RFP group, the concentration of M. tuberculosis in Guo1 and TM4 groups also reduced more obviously(P<0.05, P<0.05) than D29 group(P>0.05), while there was no significant difference between the amounts of M. tuberculosis in TM4 and Guo1 group. In conclusion, mycobacteriophage TM4 and Guo1 can lyse stationary cells of M. tuberculosis, while D29 can't, the ability of TM4 lysing stationary cells of M. tuberculosis was the same as Guo1.
柳岩, 江莉莎, 姚义勇, 曹俊, 郭述良. 噬菌体TM4、Guo1和D29对静止期结核菌裂解作用初步研究[J]. 中国人兽共患病学报, 2016, 32(1): 28-32.
LIU Yan, JIANG Li-sha, YAO Yi-yong, GUO Shu-liang. Lysis of mycobacteriophages in stationary cells of M.tuberculosis. Chinese Journal of Zoonoses, 2016, 32(1): 28-32.
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2016, Vol. 32 
