In vitro culture of Cryptosporidium parvum in HCT-8
ZHU Hui-li1, 2, LIU Li-min1, WANG Cheng-rong1, ZHANG Su-mei1, ZHANG Long-xian1, WANG Rong-jun1
1. College of Animal Science and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China; 2. College of Animal Science and Veterinary Science, Henan Institute of Science and Technology, Xinxiang 453003, China
Abstract:In order to develop in vitro infected model for Cryptosporidium parvum and to observe its developmental stages in the cells, the human ileocecal cancer (HCT-8) cells were selected to culture the parasite. The purified oocysts of C. parvum IId subtype infected HCT-8 cells in vitro, the developmental processes of C. parvum IId subtype in HCT-8 cells were observed using Giemsa staining, and the DNA of C. parvum IId subtype from different time points of infection were detected by Polymerase Chain Reaction (PCR). Following 72 h co-culture, excystation, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote and oocyst appeared orderly. Furthermore, the DNA of C. parvum IId subtype in cells were still detected at 96 h post-infection. The complete developmental processes of C. parvum IId subtype can be observed in HCT-8 cells, which provides a theoretical basis for the screening of anti-Cryptosporidium drugs and becomes a method for C. parvum IId oocyst amplification by in vitro culture.
朱惠丽, 刘利敏, 王臣荣, 张素梅, 张龙现, 王荣军. 微小隐孢子虫在HCT-8细胞内的培养[J]. 中国人兽共患病学报, 2016, 32(8): 706-710.
ZHU Hui-li, LIU Li-min, WANG Cheng-rong, ZHANG Su-mei, ZHANG Long-xian, WANG Rong-jun. In vitro culture of Cryptosporidium parvum in HCT-8. Chinese Journal of Zoonoses, 2016, 32(8): 706-710.
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