Genetic diversity of cytochrome C oxidase subunit I genes in Aedes albopictus from dengue surveillance sites in Fujian Province, China
YOU Li-bin1, ZHU Ying1, HE Wen-xiang1, WENG Yu-wei1, 2, WANG Jin-zhang1, KAN Nai-peng1, ZHANG Yong-jun1, 2
1.Fujian Center for Disease Control and Prevention, Fuzhou 350001,China; 2. Fujian Provincial Key Laboratory of Zoonosis Disease Research, Fuzhou 350001,China
Abstract：The mosquito Aedes albopictus is the primary vector for dengue virus transmission in Fujian Province. Despite that active dengue surveillance has been launched in several sites since 2005, the genetic diversity of A. albopictus from these sites remains exclusive. In this study, mosquito pools collected from dengue surveillance sites during 2015-2016 were randomly selected, the full-length mitochondrial cytochrome C oxidase subunit I (mtDNA-COI) were amplified and sequenced. Preliminary sequence alignment of 12 amplicons with the reference sequence indicated 99% homology at nucleotide level, due to variations at 9 nucleotide sites. Three haplotypes, namely H02, H03 and H08, were determined based on phylogenetic analysis with 72 reference sequences of known haplotypes. These observations facilitate surveillance and control of arboviral diseases in Fujian.
游丽斌, 朱颖, 何文祥, 翁育伟, 王金章, 阚乃鹏, 张拥军. 福建省登革热监测点白纹伊蚊mtDNA-COI基因遗传多态性研究[J]. 中国人兽共患病学报, 2017, 33(10): 872-876.
YOU Li-bin, ZHU Ying, HE Wen-xiang, WENG Yu-wei, WANG Jin-zhang, KAN Nai-peng, ZHANG Yong-jun. Genetic diversity of cytochrome C oxidase subunit I genes in Aedes albopictus from dengue surveillance sites in Fujian Province, China. Chinese Journal of Zoonoses, 2017, 33(10): 872-876.
[1] Haddad N, Mousson L, Vazeille M, et al. Aedes albopictus in Lebanon, a potential risk of arboviruses outbreak[J]. BMC Infect Dis, 2012, 12:300. doi:10.1186/1471-2334-12-300 [2] Qian QQ, Fang YL, Zhang JQ, et al. Research on the different geographic strains of Aedes albopictus [J]. Chin Frontier Health Quarant, 2016, 39(1): 66-68. doi:10.16408/j.1004-9770.2016.01.018 (in Chinese) 钱茜茜, 方义亮, 张建庆,等. 不同地理株白纹伊蚊研究现状[J]. 中国国境卫生检疫杂志, 2016, 39(1):66-68. [3] Lu BL. Fauna Sinica, Diptera: Culicidae (1, 2)[M]. Beijing: Science Press. 1997: 244. (in Chinese) 陆宝麟. 中国动物志.Ⅷ.双翅目: 蚊科(上、下)[M].北京: 科学出版社,1997: 244 [4] Chen YD. Epidemics of dengue fever in global prevalence area[J]. Chin J Epidemiol, 1990, 11(1): 51-54. (in Chinese) 陈韵冬. 登革热在全球主要流行区的流行近况[J]. 中华流行病学杂志, 1990, 11(1):51-54. [5] Hu TS, Zhang HL, Liu YH, et al. Molecular characteristics of the full-length genome of dengue serotype 1 virus strains isolated from dengue fever cases in Sino-Myanmar border region in Yunnan Province, China[J]. Chin J Zoonoses, 2017, 33(6):473-480. doi:10.3969/j.issn.1002-2694.2017.06.001(in Chinese) 胡挺松, 张海林, 刘永华等. 云南中缅边境登革1型病毒全基因组序列特征研究[J]. 中国人兽共患病学报, 2017, 33(6):473-480. [6] Lin Y, Zhang SY, Lin YY, et al. Genetic polymorphism analysis on rDNA ITS2 from different geographical strains of Aedes albopictus in Fujian Province[J]. Chin J Zoonoses, 2010,(8): 735-738. (in Chinese) 林岩, 张山鹰, 林耀莹,等. 福建省不同地理株白纹伊蚊rDNA ITS2基因多态性分析[J]. 中国人兽共患病学报, 2010,(8):735-738. [7] Huber K, Loan LL, Hoang TH, et al. Temporal genetic variation in Aedes aegypti populations in Ho Chi Minh City(Vietnam)[J]. Heredity(Edinb), 2002, 89(1): 7-14. [8] Tien TK, Vazeille-Falcoz M, Mousson L, et al. Aedes aegypti in Ho Chi Minh City (Viet Nam): susceptibility to dengue 2 virus and genetic differentiation[J]. Trans Roy Soy Trop Med Hyg, 1999, 93(6): 581-586. [9] Goubert C, Minard G, Vieira C, et al. Population genetics of the Asian tiger mosquito Aedes albopictus , an invasive vector of human diseases[J]. Heredity, 2016, 117(3): 125-134. doi:10.1038/hdy.2016.35 [10] Folmer O, Black M, Hoeh W, et al. DNA primers for amplification of mitochondrial cytochrome C oxidase subunitIfrom diverse metazoan invertebrates[J]. Mol Mar Biol Biotechnol, 1994, 3(5): 294-299. [11] Zhong D, Lo E, Hu R, et al. Genetic analysis of invasive Aedes albopictus populations in Los Angeles County, California and its potential public health impact[J]. PLoS One, 2013, 8(7): e68586. doi:10.1371/journal.pone.0068586 [12] Futami K, Valderrama A, Baldi M, et al. New and common haplotypes shape genetic diversity in Asian tiger mosquito populations from Costa Rica and Panama[J]. J Econ Entomol, 2015, 108(2):761-768. doi:10.1093/jee/tou028 [13] Tamura K, Stecher G, Peterson D, et al. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0[J]. Mol Biol Evol, 2013, 30(12): 2725-2729. doi:10.1093/molbev/mst197 [14] Fang YL, Zhang SY, Xie HG, et al. Study on mtDNA-COI genetic characteristics of Aedes albopictus in different geographic strains[J].Strait J Prev Med, 2011, 17(4): 1-4. (in Chinese) 方义亮, 张山鹰, 谢汉国,等. 不同地理株白纹伊蚊mtDNA-COI基因特征研究[J]. 海峡预防医学杂志, 2011, 17(4): 1-4. [15] Ismail NA, Dom NC, Ismail R, et al. Mitochondrial cytochrome oxidase I gene sequence analysis of Aedes albopictus in Malaysia[J]. J Am Mosq Control Assoc, 2015, 31: 305-312. doi:10.2987/moco-31-04-305-312.1 [16] Zhang RL, Yao GQ, Pan XQ, et al. Genetic diversities of different geographical populations of Aedes albopictus based on mitochondrial gene COI[J]. Chin J Zoonoses, 2017, 33(4):316-320.doi:10.3969/j.issn.1002-2694.2017.04.005 (in Chinese) 张瑞玲, 姚广琴, 潘晓倩,等. 不同地理种群白纹伊蚊线粒体基因COI的遗传多样性分析[J]. 中国人兽共患病学报, 2017, 33(4):316-320. [17] Guo S, Ling F, Wang JN, et al. Genetic polymorphism analysis of cytochrome C oxidase subunit I gene in Aedes albopictus from Zhejiang Province, China[J]. Chin J Zoonoses, 2016, 32(2):133-136. doi:10.3969/j.issn.1002-2694.2016.02.007(in Chinese) 郭颂, 凌锋, 王金娜,等. 浙江省不同地理株白纹伊蚊mtDNA-COI基因多态性研究[J]. 中国人兽共患病学报, 2016, 32(2):133-136. [18] Yang F, Zhang RL, Liu Y, et al. Genetic polymorphism analysis on mtDNA COⅠ of Aedes albopictus in Shenzhen, China[J]. Chin J Vector Biol Control, 2015, 26(4): 337-340. doi:10.11853/j.issn.1003.4692.2015.04.002 (in Chinese) 阳帆, 张仁利, 刘阳,等. 深圳市白纹伊蚊细胞色素C氧化酶亚基Ⅰ基因多态性分析[J]. 中国媒介生物学及控制杂志, 2015, 26(4): 337-340.
2017, Vol. 33 
