Genetic diversities of different geographical populations of Aedes albopictus based on mitochondrial gene COI
ZHANG Rui-ling1, 2, YAO Guang-qin2, PAN Xiao-qian2, MA De-zhen3, ZHAO Ai-hua3, ZHANG Zhong1, 2
1. Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medical University, Tai’an 271016, China; 2. Department of Pathogenic Biology, Tai’an Medical University, Tai’an 271016, China; 3. Taian Municipal Center for Disease Control and Prevention, Tai’an 271000, China
Abstract：In order to figure out phylogenetic relationship and genetic diversity of different geographical populations, genetic analyses of Aedes albopictus were performed based on mitochondrial gene COI. Based on samples collected from most distribution regions in China, mitochondrial gene Cytochrome C Oxidase Subunit I was obtained through PCR and DNA sequence. Together with some COI sequences downloaded from GenBank, 60 COI sequences with the final length of 598 bp were used for subsequent analyses. Results showed that there was no obvious divergence according to phylogenetic analyse, all sequences were clustered together in Maximum Likelihood tree. Sixteen haplotypes were detected, and four of them shared haplotypes. Haplotype diversity (Hd) was 0.737, nucleotide diversity (π) was 0.20%. Population genetic differentiation analyses demonstrated that Hainan population showed obvious divergences. In the network of haplotypes, H1 and H6 was found to be the primary haplotypes, and they formed two radical centers. All these results indicate that A. albopictus populations of China are expanding presently, and Hainan population become differential with other geographical populations, which probably attribute to geographical isolations.
张瑞玲, 姚广琴, 潘晓倩, 马德珍, 赵爱华, 张忠. 不同地理种群白纹伊蚊线粒体基因COI的遗传多样性分析[J]. 中国人兽共患病学报, 2017, 33(4): 316-320.
ZHANG Rui-ling, YAO Guang-qin, PAN Xiao-qian, MA De-zhen, ZHAO Ai-hua, ZHANG Zhong. Genetic diversities of different geographical populations of Aedes albopictus based on mitochondrial gene COI. Chinese Journal of Zoonoses, 2017, 33(4): 316-320.
[1] Beebe NW, Ambrose L, Hill LA, et al. Tracing the tiger:population genetics provides valuable insights into the Aedes ( Stegomyia ) albopictus invasion of the Australasian region[J]. PLoS Negl Trop Dis, 2013, 7(8):e2361. doi:10.1371/journal.pntd.0002361 [2] Failloux AB, Vazeille M, Rodhain F. Geographic genetic variation in populations of dengue virus vector Aedes aegypti [J]. J Mol Evol, 2002, 55:653-663. [3] Kramer LD, Ebel GD. Dynamics of flavivirus infection in mosquitoes[J]. Adv Virus Res, 2003, 60:187-232. [4] Kamgang B, Brengues C, Fontenille D, et al. Genetic structure of the tiger mosquito, Aedes albopictus , in Cameroon (Central Africa)[J]. PLoS One, 2011, 6(5):e20257. doi:10.1371/journal.pone.0020257 [5] Lin Y, Zhang SY, Lin YY, et al. Genetic polymorphism analysis on rDNA ITS2 from different geographical strains of Aedes albopictus in Fujian Province[J]. Chin J Zoonoses, 2010, 26(8):735-738. (in Chinese) 林岩,张山鹰,林耀莹,等. 福建省不同地理株白纹伊蚊rDNA ITS2基因多态性分析[J]. 中国人兽共患病学报, 2010, 26(8):735-738. [6] Guo S, Ling F, Wang JN, et al. Genetic polymorphism analysis of cytochrome C oxidase subunit I gene in Aedes albopictus from Zhejiang Province,China[J]. Chin J Zoonoses, 2016, 32(2):133-136.doi:10.3969/j.issn.1002-2694.2016.02.007(in Chinese) 郭颂,凌锋,王金娜,等. 浙江省不同地理株白纹伊蚊mtDNA-COI基因多态性研究[J]. 中国人兽共患病学报,2016,32(2):133-136. [7] Yang F, Zhang RL, Liu Y, et al. Genetic polymorphism analysis on mtDNA COI of Aedes albopictus in Shenzhen, China[J]. Chin J Vector Bio Control, 2015, 26(4):337-340.doi:10.11853/j.issn.1003.4692.2015.04.002(in Chinese) 阳帆,张仁利,刘阳,等. 深圳市白纹伊蚊细胞色素C氧化酶亚基Ⅰ基因多态性分析[J]. 中国媒介生物学及控制杂志,2015,26(4):337-340. [8] Swofford DL. PAUP:Phylogenetic analyses using parsimony, Version 4[R]. Sunderland:Sinauer & Associates, 2002. [9] Librado P, Rozas J. DnaSP v5:A software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics, 2009, 25:1451-1452. doi:10.1093/bioinformatics/btp187 [10] Excoffier L, Lischer H. Arlequin v 3.5:An integrated software package for population genetic data analysis[R]. Switzerland:Computational and molecular population genetics lab (CMPG), University of Berne, 2009. [11] Bandelt HJ, Forster P, Röhhl A. Median-joining networks for inferring intraspecific phylogenies[J]. Mol Biol Evol, 1999, 16:37-48. [12] Avise JC. Perspective:conservation genetics enters the genomics era[M]. Conservation Genetics,2010,11(2):665-669. [13] Slatkin M, Hudson RR. Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations[J]. Genetics, 1991, 129(2):555-562.
2017, Vol. 33 
