Abstract:To investigate the effect of CDR1/CDR2 or MDR1 genes overexpression on oxidative stress in Candida albicans, we evaluated the effect of H2O2 on cell viability in C. albicans overexpressing genes CDR1 /CDR2 or MDR1 and their parent strains. After establishing an oxidative stress model with H2O2, we detected reactive oxygen species (ROS),mitochondrial membrane potential (ΔΨm) and the expression of oxidative stress response-related genes (CAP1 and GRP2) and ROS clearance related-genes (SOD2 and SOD5). The results showed that C. albicans growth were inhibited by 100% after the treatment of 5 mmol/L H2O2. H2O2 caused more ROS accumulation and ΔΨm reduction in parent strains than in CDR1/CDR2 or MDR1 genes overexpressed strains (P<0.05). Compared to parent strains, the up-regulated expression of CAP1 and GRP2 were relatively less in CDR1/CDR2 or MDR1 genes overexpressed strains, moreover, the down-regulated expression of SOD2 and SOD5 were also relatively less in CDR1/CDR2 or MDR1 genes overexpressed strains (P<0.05). In conclusion, the overexpression of CDR1/CDR2 and MDR1 genes could reduce the oxidative stress response and enhance the adaptability of C. albicans to oxidative stress.
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