Bioinformatics analysis of Mycobacterium tuberculosis resistance to the macrophage autophagy mechanism by regulating miRNAs and circRNAs
ZHANG Yi-yuan1, LUO Jia-jing2, YI Zheng-jun2, FU Yu-rong1
1. College Clinical Medicine, Weifang Medical University, Weifang 261053, China; 2. Department of Clinical Laboratory, Weifang Medical University, Weifang 261053, China
Abstract:We explored the pivotal miRNAs and circRNAs which can affect the Mycobacterium tuberculosis (MTB) resistance to the human or mice macrophage autophagy, to provide the new way to find the novel tuberculosis diagnosis biomarker and therapeutic target meanwhile. We screened and filtrated the differentiated expressed autophagy related genes regulated by MTB in infected macrophage and selected the pivotal autophagy genes from the protein-protein interactions diagram and literature mining. We also integrated forecasting the relevant miRNA with that through multiple predicting databases, create the autophagy gene mRNA-miRNAs interaction diagram and selected the pivotal miRNA, verifying them by literature or experiments. Then we created miRNAs-circRNAs interactions diagram and selected the pivotal circRNA likewise. Of 18 autophagy related genes with differentiated expression in MTB infected cells were filtrated, and 16 crucial autophagy genes were predicted in the basis. Moreover four pivotal miRNAs (miR-17-5p, miR-20a-5p, miR-30a-5p, miR-33-5p) were selected which regulate autophagy by targeting mRNA of autophagy genes. In addition, six relevant potential circRNAs were selected by virtue of analysis of the connection with that four mRNAs, and speculating that has-circRNA2908 and has-circRNA6439 would be the pivotal circRNA to impair MTB resistance to cell autophagy. MiR-17-5p, miR-20a-5p, miR-30a-5p, miR-33-5p and their common upstream regulating molecules, has-circRNA2908 and has-circRNA6439 may be potential diagnosis biomarker and therapeutic target of disease.
张益源, 罗嘉婧, 伊正君, 付玉荣. 结核分枝杆菌调节miRNAs及circRNAs抗细胞自噬机制的生物分析[J]. 中国人兽共患病学报, 2018, 34(10): 888-894.
ZHANG Yi-yuan, LUO Jia-jing, YI Zheng-jun, FU Yu-rong. Bioinformatics analysis of Mycobacterium tuberculosis resistance to the macrophage autophagy mechanism by regulating miRNAs and circRNAs. Chinese Journal of Zoonoses, 2018, 34(10): 888-894.
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