Effect of multilocular cyst fluid on IDO expression in mouse bone marrow derived dendritic cells
FU Yong1,2, MENG Ru3, JIANG Tao4, ZHANG Zhuang-zhi5, WANG Hong-bin6
1.Academy of Animal and Veterinary Medicine, Qinghai University, Xining 810016, China;
2.State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China;
3.Animal Husbandry and Veterinary Station of Xining, Xining 810016,China;
4.The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;
5.Veterinary Research Institute, Xinjiang Academy of Animal Sciences,Urumqi 830000,China;
6. The Affiliated Hospital of Qinghai University, Xining 810001, China
Abstract：The objective of this study was to explore the ability of multilocular cyst fluid (MCF) to induce IDO expression in mouse bone marrow derived dendritic cells (BMDCs). BMDCs were obtained by induction culture in vitro, and coculture with MCF (MCF group), rmIFN-gamma (positive control group) and RPMI-1640 culture medium (negative control group). BMDCs of each group were collected at different time points. Real-time PCR method was used to detect IDO mRNA transcriptional level on BMDCs. Western blotting method was used to detect IDO protein level on BMDCs. ELISA method was used to detect the variation of IDO concentration in BMDCs supernatant. The results showed that MCF group of relative expression of IDO mRNA on BMDCs and IDO concentration in BMDCs supernatant reached peak value at 24 h, which were greatly higher than the negative control group(F=303.305, 5 752.473, P<0.01). While MCF group of relative expression level of IDO protein on BMDCs reached the highest value at 48 hours, which were significantly higher than that in negative control group (F=70.971, P<0.01). These results indicate that MCF has the ability to up regulate the expression of IDO on BMDCs, which provides a theoretical basis for further revealing the molecular mechanism of immune tolerance in alveolar echinococcosis.
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2018, Vol. 34 
