Molecular cloning and bioinformatics analysis on MBLAC1 of Schistosoma japonicum
ZHANG Min1,2, LIN Jiao-jiao2, HONG Yang2, FU Zhi-qiang2, HAN Hong-xiao2, QIAN Wei-feng1, LI Chang-jian2
1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China;
2. Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
Abstract:In order to clone the gene of metallo-beta-lactamases domain-containing protein 1 from Schistosoma japonicum (SjMBLAC1), and analyze the biological characteristics of the coding protein, the SjMBLAC1 gene was amplified by the polymerase chain reaction (PCR) technique with adult worm cDNA as a template,and the structural features of the coding protein were analyzed by the bioinformatics technique. Results showed that the full-length of SjMBLAC1 gene was 711 bp and it encoded a protein of 236 amino acids with a predicted molecular weight of 26 kDa and an isoelectric point of 4.84. It contained a conserved domain from the 7th to the 222nd amino acids which belonged to the MBL super family, and an N-glycosylation site at the 186th amino acid without the signal peptide and the transmembrane domain. In addition, its secondary structures comprised 8.90% α-helices, 27.97% β-sheets, and 63.14% random coils. At last, it was speculated that the SjMBLAC1 protein had 9 dominant B cell antigen epitopes. It is concluded that SjMBLAC1 was successfully cloned and analyzed by the bioinformatics technique. This study will provide the foundation for proceeding further research on the biological function of SjMBLAC1 and permit the screening new vaccine candidates against schistosomiasis.
张旻, 林矫矫, 洪炀, 傅志强, 韩宏晓, 钱伟锋, 李长健. 日本血吸虫MBLAC1基因的克隆及生物信息学分析[J]. 中国人兽共患病学报, 2018, 34(4): 315-322.
ZHANG Min, LIN Jiao-jiao, HONG Yang, FU Zhi-qiang, HAN Hong-xiao, QIAN Wei-feng, LI Chang-jian. Molecular cloning and bioinformatics analysis on MBLAC1 of Schistosoma japonicum. Chinese Journal of Zoonoses, 2018, 34(4): 315-322.
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