Influencing on development and differentiation of B cells in Mycobacterium tuberculosis infected BALB/c Mice
LIU Wen-wen1, LYU Yan1, WANG Cong1, ZHAO Wei1, WANG Tao1, BAI Li1,2
1.Basic Medical College, Dali 671000, China; 2. Yunnan Provincial Key Laboratory of Entomological Biopharmaceu-tical R&D, Dali University, Dali 671000, China
Abstract:To explore the impact on development and differentiation of B cell in BALB/c mice infected with Mycobacterium tuberculosis (Mtb), mice infected models were established with Mtb of Dali clinical isolates. After 4 weeks and 8 weeks of infected with Mtb, the bone marrow cells and splenic cells were collected from the mice, separately. The cells or cultured cells were stained by specific antibodies conjugated fluorescein. The phenotype of B cells was analyzed by flow cytometer. At the same time, mice injected with normal saline were set as control. Compared with the control group, pro-B cell(CD45R+CD43+)(4 week: t=2.886, P<0.05), immature B cell (CD45R+IgM+IgD-)(4 week: t=4.760, P<0.05) were decreased at both of the early and late stage infected with Mtb, and the mature B cells in bone marrow(CD45R+IgM+/-IgD+)(4 week: t=-3.485,P<0.05; 8 week: t=-2.594,P<0.05) and spleen (CD45R+IgMlow IgDhi) (4 week: t=-2.27,P<0.055; 8 week: t=-2.97,P<0.05)increased. Compared with the control group, expression of CD69 on murine splenic B cells increased (4 week: t=-2.271,P<0.05; 8 week: t=-2.052,P<0.05). In addition, The memory B cell (CD45R+CD27+IgD+/-)(4 week: t=-4.203,P<0.05; 8 week: t=-5.280.P<0.05) increased in the infection mice. The infection of Mtb can affect the development and differentiation of B cell, inducing differentiation of B cells into mature cells and activation of B cells, so that the body is more conducive to Mtb infection.
刘文文, 吕艳, 王聪, 赵微, 王涛, 白丽. 结核分枝杆菌感染对BALB/c小鼠B细胞发育分化的影响[J]. 中国人兽共患病学报, 2018, 34(8): 703-707.
LIU Wen-wen, LYU Yan, WANG Cong, ZHAO Wei, WANG Tao, BAI Li. Influencing on development and differentiation of B cells in Mycobacterium tuberculosis infected BALB/c Mice. Chinese Journal of Zoonoses, 2018, 34(8): 703-707.
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