Molecular epidemiology of Arctic-like rabies viruses in the Qinghai-Tibet Plateau areas of China
TIAN Deng1, LIU Gui-xiang1, SHI Yan1, JIANG Shuang-ying1, ZHAO Sheng-cang1, XU Li-li1, ZHANG Hua-yi1, LEI You-ju1, DAN Cuo2, GA Sha3, LOU Zhi-ming3
1.Qinghai Province Center for Disease Control and Prevention, Xining 810007 China; 2.Guoluo Tibentan Autonomous Prefecture Center for Disease Control and Prevention, Maqin 815000 China; 3.Yushu Tibentan Autonomous Prefecture Center for Disease Control and Prevention, Yushu 814000 China
Abstract：We investigated the epidemic trend and the characteristics of mutation about rabies virus in human and different animals in Qinghai. From 2012 to 2016, we collected 194 tissue samples from different regions of Qinghai, and used direct fluorescent antibody (DFA)to test them. The positive samples were analyzed for nucleic acid amplification and sequence analysis. We obtained 38 direct fluorescent antibody (DFA)-positive specimens from 194 tissue samples and got 8 whole genome sequence of rabies virus from these 38 positive specimens. Comparing the complete nucleotide sequences of these 8 strains with each other and with CQH2012d (KM272192.1) and 2012 CXZ1201D dog from Tibet (KC46352) strain for the homology analysis, we found that the nucleotide identity was between 95% and 100% in between any two strains. The result of the phylogenetic analysis of the N genes and G genes of these 8 strains and representative strains from China and elsewhere in the world showed that the nucleotide sequences of these viruses belong to the Arctic-like2 group, which was closely related to the China IV lineage. Analysis of the regional distribution of 38 DFA positive specimens indicated that the incidence of positive specimens gradually decreased from west to east in Qinghai Province. The Arctic-like2 was the main prevalence type of rabies in Qinghai, and its tend to expand from west to East with the migration of animals. The epidemiological data suggest that the rabies found in domestic animals or human came from infected dogs or wild wolves in the Qinghai-Tibet Plateau area in China.
田登, 刘桂香, 石燕, 姜双应, 赵生仓, 徐莉莉, 张华一, 雷有菊, 旦措, 尕沙, 罗志明. 青藏高原地区Arctic-like狂犬病病毒的分子流行病学研究[J]. 中国人兽共患病学报, 2018, 34(9): 817-822.
TIAN Deng, LIU Gui-xiang, SHI Yan, JIANG Shuang-ying, ZHAO Sheng-cang, XU Li-li, ZHANG Hua-yi, LEI You-ju, DAN Cuo, GA Sha, LOU Zhi-ming. Molecular epidemiology of Arctic-like rabies viruses in the Qinghai-Tibet Plateau areas of China. Chinese Journal of Zoonoses, 2018, 34(9): 817-822.
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2018, Vol. 34 
