Molecular epidemiology of Arctic-like rabies viruses in the Qinghai-Tibet Plateau areas of China
TIAN Deng1, LIU Gui-xiang1, SHI Yan1, JIANG Shuang-ying1, ZHAO Sheng-cang1, XU Li-li1, ZHANG Hua-yi1, LEI You-ju1, DAN Cuo2, GA Sha3, LOU Zhi-ming3
1.Qinghai Province Center for Disease Control and Prevention, Xining 810007 China; 2.Guoluo Tibentan Autonomous Prefecture Center for Disease Control and Prevention, Maqin 815000 China; 3.Yushu Tibentan Autonomous Prefecture Center for Disease Control and Prevention, Yushu 814000 China
Abstract:We investigated the epidemic trend and the characteristics of mutation about rabies virus in human and different animals in Qinghai. From 2012 to 2016, we collected 194 tissue samples from different regions of Qinghai, and used direct fluorescent antibody (DFA)to test them. The positive samples were analyzed for nucleic acid amplification and sequence analysis. We obtained 38 direct fluorescent antibody (DFA)-positive specimens from 194 tissue samples and got 8 whole genome sequence of rabies virus from these 38 positive specimens. Comparing the complete nucleotide sequences of these 8 strains with each other and with CQH2012d (KM272192.1) and 2012 CXZ1201D dog from Tibet (KC46352) strain for the homology analysis, we found that the nucleotide identity was between 95% and 100% in between any two strains. The result of the phylogenetic analysis of the N genes and G genes of these 8 strains and representative strains from China and elsewhere in the world showed that the nucleotide sequences of these viruses belong to the Arctic-like2 group, which was closely related to the China IV lineage. Analysis of the regional distribution of 38 DFA positive specimens indicated that the incidence of positive specimens gradually decreased from west to east in Qinghai Province. The Arctic-like2 was the main prevalence type of rabies in Qinghai, and its tend to expand from west to East with the migration of animals. The epidemiological data suggest that the rabies found in domestic animals or human came from infected dogs or wild wolves in the Qinghai-Tibet Plateau area in China.
田登, 刘桂香, 石燕, 姜双应, 赵生仓, 徐莉莉, 张华一, 雷有菊, 旦措, 尕沙, 罗志明. 青藏高原地区Arctic-like狂犬病病毒的分子流行病学研究[J]. 中国人兽共患病学报, 2018, 34(9): 817-822.
TIAN Deng, LIU Gui-xiang, SHI Yan, JIANG Shuang-ying, ZHAO Sheng-cang, XU Li-li, ZHANG Hua-yi, LEI You-ju, DAN Cuo, GA Sha, LOU Zhi-ming. Molecular epidemiology of Arctic-like rabies viruses in the Qinghai-Tibet Plateau areas of China. Chinese Journal of Zoonoses, 2018, 34(9): 817-822.
[1] Wang LH, Tang Q,Liang GD,et al.Rabies and rabies virus in wildlife in mainland China, 1990-2013[J]. Internat J infect Dis, 2014, 8(25):22-129.DOI:org/10.1016/j.ijid.2014.04.016 [2] Zhang YZ, Xiong CL, Xiao DL,et al.Human rabies in China[J].Emerg Infect Dis, 2005,11: 1983-1984.DOI:10.3201/eid1112.040775 [3] Hu RL,Tang Q, Tang JR, et al.Rabies in China: an update[J].Vector-Borne Zoonoat Dis,2009, 9: 1-11.DOI:org/10.1089/vbz.2008.0046 [4] 张永振,熊成龙,邹洋,等.贵州省安龙及其周边地区狂犬病流行病学研究[J].中国病毒学, 2006, 21(4):395-440. [5] 王纯山,袁有,田玉生,等.疯狼引起两次狂犬病调查[J].中国人兽共患病杂志, 1997, 13(1):55-57. [6] 施柏年,周建方,俞涧,等. 鼬獾咬伤致狂犬病7例[J].中国人兽共患病杂志,2004, 20(12):1103. [7] 雷永良,王晓光,陶晓燕,等.浙江地区鼬獾和犬源狂犬病病毒分离株全基因组测序与分析[J].中国病毒学杂志,2010, 26(1):45-52. [8] Shao XQ, Yan XJ, Luo G.L,et al. Genetic evidence for domestic raccoon dog rabiescaused by Arctic-like rabies virus in Inner Mongolia, China[J].Epidemi Infect,2011,139(4):629-635.DOI:10.1017/S0950268810001263 [9] Rahman A, Sobia P, Gupta N, et al.Mycobacterium tuberculosis subverts the TLR-2-MyD88 pathway to facilitate its translocation into the cytosol[J]. PLoS One, 2014, 9(1): e86886. DOI:10.1371/journal.pone.0086886 [10] Chomczynski P.A reagent for the single-step simultaneous isolation of RNA,DNA and proteins from cell and tissue samples[J].BioTechniques, 1993, 15, 532-537. [11] Chomczynski P, Sacchi N.Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction[J].Anal Biochem, 1987, 162:156-159.DOI:10.1006/abio.1987.9999 [12] 江禹, 王莉莉,韩小虎,等. 动物狂犬病巢式RT-PCR方法的检测方法的建立[J].中国兽医学报, 2009, 29(8):1003-1007. [13] Tao XY, Tang Q, Simon RN,et al, Molecular phylodynamic analysis indicates lineage displacement occurred in Chinese rabies epidemics between 1949 to 2010[J]. PLoS Neglected Tropical Diseases, 2013,7(7):1-13.DOI:org/10.1371/journal.pntd.0002294 [14] Hyun BH, Kyoung KL, In JK, et al.Molecular epidemiology of rabies virus isolates from South Korea[J]. Virus Research,2005,114:113-125. DOI:org/10.1016/j.virusres.2005.06.004 [15] Zhang YZ, Xiong CL, Lin XD, et al.Genetic diversity of Chinese rabies viruses: evidence for the presence of two distinct clades in China[J]. Infect Genet Evolu,2009, 9:87-96.DOI:org/10.1016/j.meegid.2008.10.014 [16] 赵丽. 世界各国如何防控狂犬病[J].中国动物检疫, 2006, 23(11): 48-49. [17] Sharon LM, Jean SS, Lillian AO, et al, Emerging pattern of rabies deaths and increased viral infectivity[J].Emerg Infect Dis,2003,4(9):151-154.DOI: 10.3201/eid0902.020083 [18] Mansfield Kl, Racloz V, McElhinney LM, et al.Molecular epidemiological study of Arctic rabies virus isolates from Greenland and comparison with isolates from throughout the Arctic and Baltic regions[J].Virus Res,2006,116: 1-10.DOI:org/10.1016/j.virusres.2005.08.007 [19] Jae-Ku Oem, seong-Hee Kim, Yeon-Hee Kim,et al.Reemergence of rabies in the southern han river region,Korea[J].J Wildlife Dis, 2014,50(3) : 681-688.DOI:org/10.7589/2013-07-177 [20] Luzia Helena Queiroz, Silvana Regina Favoretto, Elenice Maria S.Cunha, et al. Rabies in southeast Brazil: A change in the epidemiological pattern[J]. Archives Virol,2012,157(1):93-105.DOI: 10.1007/s00705-011-1146-1 [21] Meng SL, Yan JX, Xu GL, et al.Molecular epidemiological study targeting the glycoprotein gene of rabies virus isolates from China[J]. Virus Res. 2007, 124: 125-138.DOI:org/10.1016/j.virusres.2006.10.011 [22] Mansfield Kl, Racloz V, McElhinney LM, et al. Molecular epidemiological study of Arctic rabies virus isolates from Greenland and comparison with isolates from throughout the Arctic and Baltic regions[J].Virus Research,2006,116:1-10. DOI:org/10.1016/j.virusres.2005.08.007 [23] Kuzmin IV, Hughes GJ, BotvInkin AD, et al.Arctic and arctic like rabies viruses :distribution, phylogeny and evolutionary history[J]. Epidemiol Infect, 2008, 136, 509-519.DOI: 10.1017/S095026880700903X [24] Johnson N, Black C, Smith J, et al,Rabies emergence among foxes in Turkey[J]. J Wild Dis, 39:262-270.DOI:org/10.7589/0090-3558-39.2.262 [25] 王力华,唐青,梁国栋,等.野生动物在狂犬病中的作用[J].中国动物检疫,2014, 31(6):39-42.