Abstract：In order to prepare the monoclonal antibodies against Zika virus NS1 protein, a recombinant plasmid containing Zika virus NS1 gene was constructed, and the recombinant NS1 protein was expressed by E.coli. The mice were immunized with the purified recombinant NS1 protein, and anti-NS1 monoclonal antibodies were prepared by cell fusion and screening. After rounds of selection, three strains of hybridoma cell lines which secreted monoclonal antibodies against Zika virus NS1 protein were established and designated as 3E2, 6B8 and 7D11 respectively. The monoclonal antibodies with high purity were generated and purified from the ascites. All the mAbs belong to IgG 1 isotype, and have a specific reaction to the recombinant NS1 protein. In conclusion, the monoclonal antibodies against NS1 were prepared, which will provide a raw material for the ELISA detection method of Zika virus.
赵亭亭, 徐叶, 廖旻晶, 刘如石, 李晓丹. 寨卡病毒NS1蛋白的原核表达和单克隆抗体的制备[J]. 中国人兽共患病学报, 2019, 35(3): 196-200.
ZHAO Ting-ting, XU Ye, LIAO Min-jing, LIU Ru-shi, LI Xiao-dan. Prokaryotic expression and monoclonal antibody preparation against NS1 protein of Zika virus. Chinese Journal of Zoonoses, 2019, 35(3): 196-200.
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2019, Vol. 35 
