Construction of drosophila Salmonella death model and its assessment
GENG Shi-zhong, XI Jun-meng, YANG Jia-xing, SUN Da-quan, WEI You-heng, PAN Zhi-ming, JIAO Xin-an
Key Laboratory of Prevention and Control of Biological Hazard Factors(Animal Origin) for Agrifood Safety and Quality,Ministry of Agriculture of China/Jiangsu Key Laboratory of Zoonosis, Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China
Abstract:To compare the virulence of different Salmonella in the Drosophila, construction of Drosophila/Salmonella infection model and its assessment were made. S.typhimurium SL1344, 14028S and S.enteritidis C50041 and its mutant strain C50041ΔspiC infected 30 CS and yw drosophilas, respectively, at an OD600 of 100 by feeding or acupuncture, live Drosophilas were numbered every 3 hours for survival rate to compare Salmonella virulence. The results showed that after being infected S.enteritidis C50041 and C50041 ΔspiC by feeding for 120 h, the survival rate of 30 CS Drosophilas was 0%, and the survival rate of yw Drosophilas was less than 20%, but the survival rate of CS and yw drosophilas infected S.enteritidis by acupuncture were over 80%; the survival rate of CS and yw drosophilas infected S.typhimurium by feeding was over 90%, the survival rate of that by acupuncture is over 60%. Comparative analysis showed that S.typhimurium intestinal-infection was resistant and S.enteritidis intestinal-infection was sensitive to drosophilas, there were significant differences; S.enteritidis virulence by intestinal infection was significantly higher than that by the non-intestinal infection to drosophilas, there was no significant difference between CS and yw drosophilas. In this study, Drosophila/Salmonella infection model was constructed successfully and could assess the virulence of different Salmonella, the sensitive intestinal-infection model of S.enteritidis C50041 by feeding Drosophila will provide a basis for the subsequent identification of the functional genes of S.enteritidis for its pathogenic mechanism.
耿士忠, 席俊萌, 杨家兴, 孙大泉, 韦有恒, 潘志明, 焦新安. 沙门菌果蝇感染致死模型构建及其评价[J]. 中国人兽共患病学报, 2019, 35(4): 311-314.
GENG Shi-zhong, XI Jun-meng, YANG Jia-xing, SUN Da-quan, WEI You-heng, PAN Zhi-ming, JIAO Xin-an. Construction of drosophila Salmonella death model and its assessment. Chinese Journal of Zoonoses, 2019, 35(4): 311-314.