Abstract:We investigated the Candidatus Neoehrlichia mikurensis(Nm) infection in wild rodents and to make clear the genetic characteristics of the agents from forest areas in northeastern China. Nested PCR amplification, following by sequence analysis, was carried out. The sequences of groEL gene fragment amplified from rodents were compared with corresponding part of the sequences deposited in GenBank. A total number of 181 rodents were tested, including 67 in Ji’an of Jilin Province, 66 in Kuandian of Liaoning Province and 48 in Mishan of Heilongjiang province. The positive rates were 2.76% (5/181), and for Jin’an, Kuandian and Mishan were 5.97% (4/67), 1.52% (1/66) and 0%(0/48) , respectively. There were not significant differences in different regions and in different mouse species for their infection with Nm. Phylogenetic analysis based on groEL nucleotide sequences revealed the 5 positive samples that correspond with genetype ClusterⅠ. It’s concluded that the nature focus of Nm did exist in the area of northeastern China.
王卓, 王建伟, 于淼, 冯立, 王秀红, 许志伟, 田圃, 吴益民. 东北边境地区啮齿动物中新埃立克体感染调查及groEL基因序列分析[J]. 中国人兽共患病学报, 2019, 35(4): 330-333.
WANG Zhuo, WANG Jian-wei, YU Miao, FENG Li, WANG Xiu-hong, XU Zhi-wei, TIAN Pu, WU Yi-min. Investigation on Candidatus Neoehrlichia mikurensis in rodents collected in forest area in the northeastern region of China. Chinese Journal of Zoonoses, 2019, 35(4): 330-333.
[1] Cornelia S, Relja B, Jose AO, et al.Neoehrlichiosis: an emerging tick-borne zoonosis caused by Candidatus Neoehrlichia mikurensis[J]. Exp Appl Acarol, 2015, 6, 17. DOI: 10.1007/s10493-015-9935-y [2] Kawahara M, Rikihisa Y, Isogai E, et al.Ultrastructure and phylogenetic analysis of Candidatus Neoe-hrlichia mikurensis in the family Anaplasmataceae, isolated from wild rats and found in Ixodes ovatus ticks[J]. Int J Syst Evol Microbiol, 2004, 54(5): 1837-1843. DOI: 10.1099/ijs.0.63260-0 [3] Schouls LM, Van DP I, Rijpkema SG, et al.Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato, and Bartonella species in Dutch Ixodes ricinus ticks[J]. J Cli Microbiol,1999, 37(7): 2215-2222. [4] Obiegala A, Pfeffer M, Pfister K, et al.Candidatus Neoehrlichia mikurensis and Anaplasma phagocytophilum: prevalences and investigations on a new transmission path in small mammals and ixodid tick[J]. Parasit Vectors, 2014, 7: 563. DOI: 10.1186/s13071-014-0563-x [5] Silaghi C, Woll D, Mahling M, et al.Candidatus Neoehrlichia mikurensis in rodents in an area with sympatric existence of the hard ticks Ixodes ricinus and Dermacentor reticulatus, Germany[J]. Parasit Vectors, 2012, 5: 285. [6] Rar VA, Livanova NN, Panov VV, et al.Genetic diversity of Anaplasma and Ehrlichia in the Asian part of Russia[J]. Ticks Tick Borne Dis, 2010, 1(1): 57-65. DOI: 10.1016/j.ttbdis.2010.01.002 [7] Li H, Jiang JF, Liu W, et al.Human infection with Candidatus Neoehrlichia mikurensis, China[J]. Emerg Infect Dis, 2012, 18(10): 1636-1639. DOI: 10.3201/eid1810.120594 [8] Li H, Jiang J, Tang F, et al.Wide distribution and genetic diversity of “Candidatus Neoehrlichia mikurensis” in rodents from China[J]. Appl Environ Microbiol, 2013, 79(3): 1024-1027. DOI: 10.1128/AEM.02917-12 [9] 吴益民, 张志强, 王洪军, 等. 东北地区远东蜱传斑点热的流行病学调查[J]. 中华流行病学杂志, 2008, 29(12): 1173-1175. DOI: 10.3321/j.issn:0254-6450.2008.12.001 [10] 王卓, 王茸, 周德壮, 等. 东北林区啮齿动物中蜱媒立克次体的检测及复合感染研究[J]. 中国媒介生物学和控制杂志, 2015, 26(5): 461-464. DOI: 10.11853/j.issn.1003.4692.2015.05.009 [11] Fang LQ, Liu K, Li XL, et al.Emerging tick-borne infections in mainland China: an increasing public health threat[J]. Lancet Infect Dis, 2015, 15(12): 1467-1479. DOI: 10.1016/s1473-3099(15)00177-2 [12] Yabsley MJ, Murphy SM, Luttrell MP, et al.Characterization of Candidatus Neoehrlichia lotoris (family Anaplasmataceae) from raccoons (Procyon lotor)[J]. Int J Syst Evol Microbiol, 2008, 58(12): 2794-2798. DOI: 10.1099/ijs.0.65836-0 [13] Pan H, Liu S, Ma Y, et al.Ehrlichia-like organism gene found in small mammals in the suburban district of Guangzhou of China[J]. Ann N Y Acad Sci, 2003, 990: 107-111. [14] Christina WO, Eva K, Krista V, et al.First case of human “Candidatus Neoehrlichia mikurensis” infection in a febrile patient with chronic lymphocytic leukemia[J]. J Clin Microbiol, 2010, 48(5): 1956-1959. DOI: 10.1128/JCM.02423-09 [15] 王卓, 吴琼, 杨慎江, 等.长白山地区蜱类和鼠类中查菲埃立克体感染调查及16S rRNA序列分析[J]. 中国病原生物学杂志, 2017, 12(10): 971-974. DOI: 10.13350/j.cjpb.171011 [16] Burri C, Schumann O, Schumann C, et al.Are Apodemus spp. mice and Myodes glareolus reservoirs for Borrelia miyamotoi, Candidatus Neoehrlichia mikurensis, Rickettsia helvetica, R. monacensis and Anaplasma phagocytophilum?[J]. Ticks Tick Borne, 2014, 5(3): 245-251. DOI: 10.1016/j.ttbdis.2013.11.007