Epidemiological characteristic of human brucellosis and MLVA genotyping for Brucella isolates in Fujian,China,2008-2017
HAN Teng-wei1, LIN Dai-hua1, LIU Jing1, LIU Wei-jun1, XIAO Fang-zhen1, CHEN Zhi-ping2, DENG Yan-qin1, 2
1.Fujian Center for Disease Control and Prevention, Fujian Provincial Key Laboratory for Zoonosis Research,Fuzhou 350001,China; 2.College of Public Health, Fujian Medical University, Fuzhou 350122, China
Abstract：To explore the epidemiological characteristics of human brucellosis in Fujian Province and the typing of Brucella isolates, the data of brucellosis cases during 2008-2017 were extracted from China Information System for Disease Control and Prevention and analyzed; molecular biological methods(BCSP31-PCR,AMOS-PCR and MLVA-16)were used for Brucella typing. The results showed that during 2008-2017, the incidence of brucellosis in Fujian increased year by year with an annual incidence of 0.11/105; a total of 416 cases were reported and distributed sporadically in 70 counties (80%) in the province; the peak of brucellosis occurred from April to August; 261 (62.7%) cases aged 40-64; the highest incidence rate was at age group 60-64 years old (0.27/105); the incidence ratio of male to female was 2.50:1; farmers and herdsmen accounted for 211(50.7%) cases. With classical biotyping and molecular genotyping, two species (B. melitensis and B.suis) and two biovars (B. melitensis biovar 3, n=35; and B. suis biovar 3, n=5) were identified in the 40 Brucella isolates, and B. melitensis species (35, 87.5%) were dominant in Fujian Province. MLVA-16 revealed 28 genotypes in 35 B. melitensis isolates and 4 genotypes in 5 B. suis isolates respectively, including 26 unique and 6 shared genotypes (14 isolates, 35.0%). Comparison with 147 isolates from other provinces showed that 4 genotypes of B. melitensis in Fujian were shared with that in Inner Mongolia or Guangdong. And some isolates were genetically closed with isolates in other provinces, mainly concentrated in panel 2B, with differences only in 1-3 sites. Our study indicates that the epidemic intensity of human brucellosis in Fujian increased yearly. It is necessary to strengthen surveillances and take corresponding measures in high-risk population for brucellosis control and prevention. MLVA-16 revealed highly genetic polymorphism in Fujian Brucella isolates suqqests that this method is a useful tool for genetic diversity analysis and molecular epidemiology trace-back investigations in brucellosis surveillance.
韩腾伟, 林代华, 刘菁, 刘维俊, 肖方震, 陈志平, 邓艳琴. 福建省2008-2017年人间布鲁氏菌病流行特征及分离株MLVA分型研究[J]. 中国人兽共患病学报, 2019, 35(5): 382-388.
HAN Teng-wei, LIN Dai-hua, LIU Jing, LIU Wei-jun, XIAO Fang-zhen, CHEN Zhi-ping, DENG Yan-qin. Epidemiological characteristic of human brucellosis and MLVA genotyping for Brucella isolates in Fujian,China,2008-2017. Chinese Journal of Zoonoses, 2019, 35(5): 382-388.
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2019, Vol. 35 
