Application of Multi-locus sequence typing in the genetic evolution of Brucella clinical strains isolated in Xinjiang
LI Bo1, YUE Xi-hong1, CUI Bu-yun2, LI Wei1, LIU Zhi-guo3, SHANG Xiu-jian1
1.Xinjiang Uygur Autonomous Regional Center for Disease Control and Prevention,Urumqi 830002,China; 2.National Institute of Infections Disease Control and Prevention,Chinese Center for disease Control and Prevention,Beijing 102206,China; 3.Ulanqab Center for Endemic Disease Control,Ulanqab 012000,China
Abstract：The aims of our study is to investigate the application of Multi-locus sequence typing in the genetic evolution of clinical Brucella strains isolated and understand the population structure and genetic evolution of isolated strains. A total 24 strains from 7 prefectures of Xinjiang in 2015, 2016 and 3 standard reference strains were analyzed by MLST to determine sequences types, BioNumerics was used to constructed minimum spanning tree of isolated strains. We collected the MLST results of 186 strains from different provinces in refer to the former research in order to analysis on distribution features of STs. Results showed that 24 strains of Brecella clinical isolated were all ST8, 3 standard reference strains were ST7,ST1,ST14, respectively. The results showed that 9 MLST loci of 24 strains were exactly the same, and the populaion structure of isolated strains was single. The MLST results of 186 strains showed that ST8 type was prevalent in China and most populations of north. The MLST method could not be used to differentiate biotype of Brucella. In conclusion, the sequence type of ST8 is prevalent in Xinjiang, MLST is only a supplementary method for species construction and evolutionary research of Brucella.
李博, 岳锡宏, 崔步云, 黎唯, 刘志国, 尚修建. 多位点序列分型在新疆人间布鲁氏菌病临床分离株遗传进化研究中的应用[J]. 中国人兽共患病学报, 2019, 35(5): 411-415.
LI Bo, YUE Xi-hong, CUI Bu-yun, LI Wei, LIU Zhi-guo, SHANG Xiu-jian. Application of Multi-locus sequence typing in the genetic evolution of Brucella clinical strains isolated in Xinjiang. Chinese Journal of Zoonoses, 2019, 35(5): 411-415.
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2019, Vol. 35 
