Pathogenicity in BALB/c mice infection with Toxoplasma strain deficient in ROP16I/III
HE Jia-li, CHEN Shou-bin, CUI Wen, WANG Cong, LUO Qing-li, SHEN Ji-long, WANG Wei
School of Basic Medical Sciences, Anhui Medical University, Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Pathogen Biology And Anhui Key Laboratory of Zoonoses, Hefei 230032, China
Abstract:This study aims to explore the virulence and pathogenicity of ROP16, a rhoptry effector molecule of T. gondii. BALB/c mice were infected with wild type and ROP16I/III gene knockout RH strain of T. gondii. Clinical signs and survival time were observed and histopathology of brain and lung tissues were examined by HE staining. Cytokines of splenocyte in infected animals were detected by qRT-PCR. No significant difference was observed in the pathogenicity between the two groups. HE staining showed no remarkable pathology difference in lung and brain tissues between the two groups of mice. Additionally, deletion of the effector ROP16I/III did not impact expressed level of IL-10, IL-12, TNF-α and IFN-γ mRNA(P>0.05)except for the elevated level of Arg-1 mRNA (P<0.05). ROP16I/III of type I strain of T.gondii may not be only, if any, the virulence-associated molecule during acute infection. ROP16I/III enhance the Arg-1 expression, which is involved of the early proliferation in host cells.
何佳丽,陈守斌,崔雯,王聪,罗庆礼,沈继龙,王维. 弓形虫RH株ROP16I/III基因缺陷虫株感染BALB/c鼠的致病性研究[J]. 中国人兽共患病学报, 2019, 35(7): 620-625.
HE Jia-li, CHEN Shou-bin, CUI Wen, WANG Cong, LUO Qing-li, SHEN Ji-long, WANG Wei. Pathogenicity in BALB/c mice infection with Toxoplasma strain deficient in ROP16I/III. Chinese Journal of Zoonoses, 2019, 35(7): 620-625.
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