Abstract:Our study aimed to use the B. subtilis spores as vaccine antigen presenting vectors. We constructed a recombinant B. subtilis spores that can display recombinant Yersinia Pestis F1 antigen on the surface, then analyzed the immunogenicity and protective efficacy of the vaccine by performing animal experiments. The CotB gene from B. subtilis was inserted into pDG1664 integrational vector followed by ligation with F1 antigen gene; and then recombinant plasmids were integration into the genome of B. subtilis PY-79 by genetic homologous recombination. The methods of PCR, Western blot were used to identify the expression of F1 proteins presented on the surface of B. subtilis spores. Mice were immunized orally without adjuvants, then analyzed immunization by ELISA. The results showed that we successfully constructed the recombinant strains of B. subtilis which was integrated with F1 gene, and F1 proteins could be presented successfully on the surface of spores. Immunization of the recombinant spores can elicit high titer of specific IgG antibodies in mice. Above all, our study successfully recombined a B. Subtilis Spore-Based Plague Vaccine which could stably express F1 antigen on the spore coat, and mouse which was immunized with the recombinant B. subtilis could induce effective immune response. The recombinant spores can be used as a new type of plague candidate vaccine based on B. subtilis spore display system.
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