Molecular detection of spotted fever group rickettsiae-infected ticks collected from yaks in Shiqu County of Sichuan Province, China
LIU Cheng-cheng1, TANG Tian-cai1, TA Ying2, LIN Bao-shan2, YUAN Dong-bo3, GUO Li3, HOU Wei3, MO Xi3, YANG Ai-guo3, HAO Li-li1, LI Rui4
1. College of Life Science & Technology, Southwest Minzu University, Chengdu 610041, China;; 2. Center for Animal Disease Control and Prevention in Abazhou,Maerkang 624000, China; 3. Center for Animal Disease Control and Prevention in Sichuan Province,Chengdu 610041, China; 4. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Abstract:In order to investigate tick species and the percentage of spotted fever group rickettsiae positive in ticks collected from yaks in Shiqu County, Sichuan Province, the ticks were first classified by morphological identification. The total DNA of ticks was extracted and partial sequences of 16S rRNA of ticks, ompA and ompB gene of SFG rickettsia were amplified by PCR,respectively. The positive products were sequenced and compared through the NCBI database and phylogenetic trees were constructed based on 16S rRNA, ompA and ompB for determination of ticks species and spotted fever group rickettsia, respectively. A total of 818 ticks were collected from 4 villages in Shiqu County and only Dermacentor everestianus (78.97%, 646/818) and Haemphysalis qinghaiensis (21.03%, 172/818) were found. The total infection rate of SFG rickettsia in ticks was 49.8%. Four sequences of ompA (Uncultured Rickettsia sp.shiqu1, R.raoultii.shiqu2, R.raoultii.shiqu3 and R.raoultii.shiqu4) and four sequences of ompB (Uncultured Rickettsia sp.shiqu1, R.raoultii.shiqu2, R.raoultii.shiqu3 and R.raoultii.shiqu4) were obtained. Phylogenetic analysis showed that: for ompA, uncultured Rickettsia sp.shiqu1 had the closest relationship with the uncultured Rickettsia sp.(MG228270)isolated from Qinghai; for ompB, uncultured Rickettsia sp.shiqu1 had the closest relationship with the uncultured Rickettsia sp.(KC888953)and Candidatus Rickettsia longicornii(MG906675)isolated from Korea. R.raoultii.shiqu2-4 for ompA and ompB gene had the closest relationship with the human pathogenic R.raoultii. In this study, R.raoultii was first detected in D.everestianus from yaks. At present, only D. everestianus and H. qinghaiensis were found in Shiqu with high infection rate of R. raoultii and there exists a high risk of human infection.
刘城成, 唐天才, 塔英, 林宝山, 袁东波, 郭莉, 侯巍, 莫茜, 阳爱国, 郝力力, 李锐. 四川石渠县牦牛源蜱感染斑点热群立克次体分子流行病学调查[J]. 中国人兽共患病学报, 2020, 36(1): 50-55.
LIU Cheng-cheng, TANG Tian-cai, TA Ying, LIN Bao-shan, YUAN Dong-bo, GUO Li, HOU Wei, MO Xi, YANG Ai-guo, HAO Li-li, LI Rui. Molecular detection of spotted fever group rickettsiae-infected ticks collected from yaks in Shiqu County of Sichuan Province, China. Chinese Journal of Zoonoses, 2020, 36(1): 50-55.
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