Biological characteristics of Listeria monocytogenes ST145 strain isolated from of a lactation diarrhea case
LIU Li1, ZHANG Jing-rui1, WANG Yi-qian2, YANG Ru-gang1, XU Cai-hong1, LIU Jing-jing1,YE Chang-yun2
1. Clinical Laboratory, the Fourth Hospital of Shijiazhuang (Shijiazhuang Obstetrics and Gynecology Hospita), Shijiazhuang 050000, China; 2. State Key Labooratory for Infectious Disease Prevention and Control, National Institute for Commumicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Abstract:We aim to investigate the biological characteristics of the Listeria monocytogenes from a case of lactation diarrhea, including molecular characteristics and virulence gene carrying the strain. The clinical information of patient was collected. The isolated strain was identified by VITEK- 2 COMPACT30 automatic bacterial analyzer identification system, and characterized by serotyping, pulsed-field gel electrophoresis (PFGE) and multi locus sequence typing (MLST). The virulence genes was detected by PCR method. The five antibiotics susceptibility of the strain was tested by E-test method. Results showed that postpartum lactation patient with unrestricted diarrhea was caused by L. monocytogenes, who had eaten muskmelon and refrigerated yogurt. The isolate was belonged to serotype 4b and ST145, carrying the main virulence genes (plcB, act, hly, iap, prfA and inlA) and was sensitive to penicillin, ampicillin,mer openem, erythromycin and cotrimoxazol. The detection of L. monocytogenes in stool samples from patients with diarrhea in high-risk populations is important, which would be helpful for the timely treatment and prevention of poor prognosis. Further study about the listeriosis caused by this particular type strain is needed to be carried in China, including its possible source and infection risk.
张敬蕊, 刘力, 王怡倩, 杨如刚, 许彩虹, 刘菁菁, 叶长芸. 1例产后哺乳期腹泻患者分离单增李斯特菌的生物学特征分析[J]. 中国人兽共患病学报, 2020, 36(2): 169-172.
LIU Li, ZHANG Jing-rui, WANG Yi-qian, YANG Ru-gang, XU Cai-hong, LIU Jing-jing,YE Chang-yun. Biological characteristics of Listeria monocytogenes ST145 strain isolated from of a lactation diarrhea case. Chinese Journal of Zoonoses, 2020, 36(2): 169-172.
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