1. Fujian Center for Disease Control and Prevention, Fujian Provincial Key Laboratory for Zoonoses Research, Fuzhou 350001, China; 2. School of Public Health, Fujian Medical University, Fuzhou 350108, China
Abstract：To explore genomic characteriation of 2019-nCoV,throat swab specimens from COVID-19 patients were inoculated on Vero-E6 cells.Six days post-inoculation,supernatants of cell cultures were collected, tested, and sequenced on the Ion Torrent S5 Next-Generation Sequencing system. Two strains viruses were isolated from 12 specimens (eight of them were positive in ORF1ab gene and N gene, one of them was positive in N gene and three of them were negative in ORF1ab gene and N gene ), as confirmed by online BLAST search at NCBI website. Genetic similarity of more than 99.9% were observed, from genomic level to structure genes including E,M,N and S genes, between the 2 isolates and the 2019-nCoV reference strain Wuhan-Hu-1 that isolated from Wuhan, Hubei province. Therefore, these results indicated that 2019-nCoV isolates in Fujian province have not mutated significantly yet so far.
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2020, Vol. 36 
