Abstract:To comparatively analyze the detection effect of viral nucleic acid in throat swab and sputum sample of 2019-nCoV cases. GAPDH housekeeping genes, viral ORF 1ab genes, N genes, and S genes were tested and compared by real-time RT-PCR in throat swabs and sputum specimens from 4 confirmed cases of 2019-nCoV. In the throat swabs and sputum samples of 4 cases, the human cell housekeeping gene GAPDH showed obvious and typical amplification signal curves. In the detection of viral ORF 1ab gene, N gene, and S gene, the amplification signal of sputum samples was stronger than that of throat swab, and the CT value of amplification curve was lower than that of throat swab, especially in case 1 and case 4, and the throat swab of case 4 showed negative results using commercial real-time RT-PCR kits, while the sputum specimens showed clear positive results. Therefore, in the viral nucleic acid testing of 2019-nCoV laboratory detection, the virus content in sputum specimens was higher than that in throat swab specimens, and the detection effect was better than that in throat swab specimens.
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