The different role of NLRP3 inflammasome in the early stage of pneumonia caused by influenza A virus and MRSA in mice
SHI Yun-feng1, SHI Xiao-han1, BA Jun-hui1, CHEN Jian-ning2, LUO Jin-mei1, WU Ben-quan1
1. Department of MICU, Department of Pulmonary and Critical Care Medcine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; 2. Department of Pathology, the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China
Abstract：To evaluate and compare the role of NLRP3 inflammasome between pneumonia caused by influenza A virus H1N1(IAV H1N1) and methicillin-resistant Staphylococcus aureus(MRSA)in mice. Pneumonia caused by intranasal inoculation with IAV H1N1 FM1 strain and MRSA in mice was established respectively. After infected for 24 h, fluorescence quantitative PCR was applied to detect the expression of NLRP3 and IL-1β mRNA in lung,Western blot was used to detect the protein of NLRP3 in lung,and the concentration of IL-1β in serum was measured by enzyme linked immunosorbent assay(ELISA). The transcription and translation levels of NLRP3 were significantly different among control, H1N1 and MRSA groups (F=38.782 and 1 325.64, P=0.000). The transcription level of IL-1 in lung and concentration of IL-1 in serum were also significantly different among control, H1N1 and MRSA groups (F=1 253.97 and 41.974, respectively, P=0.000 and 0.000 respectively). In the IAV H1N1 group, the expression of NLRP3 mRNA and NLRP3 proteins were significantly higher than those in the control group and MRSA group(all P<0.05).The expression of IL-1β mRNA and concentration of IL-1β in H1N1 group were significantly higher than those in the control group(P<0.01). The expression of NLRP3 mRNA in MRSA group was higher than control group(P<0.05),while, the expression of NLRP3 protein between MRSA group and control group wasn’t noted. In the MRSA group, the expression of IL-1β mRNA and concentration of IL-1β in serum were significantly higher than those in the control group and H1N1 group(all P<0.01).It suggests that NLRP3 inflammasome involve in pneumonia induced by influenza A virus H1N1,but it do not play an improtant role in the intense inflammatory response caused by MRSA in early stage.
石云锋, 师小函, 巴俊慧, 陈健宁, 罗进梅, 吴本权. NLRP3炎症小体在甲型流感病毒和MRSA致小鼠肺炎早期的作用差异[J]. 中国人兽共患病学报, 2020, 36(7): 566-571.
SHI Yun-feng, SHI Xiao-han, BA Jun-hui, CHEN Jian-ning, LUO Jin-mei, WU Ben-quan. The different role of NLRP3 inflammasome in the early stage of pneumonia caused by influenza A virus and MRSA in mice. Chinese Journal of Zoonoses, 2020, 36(7): 566-571.
2020, Vol. 36 
