Abstract：To investigate the related mechanism of dendritic cells(DCs) regulating Thl immune response during early Plasmodium chabaudi chabaudi AS(P.c.chabaudi AS) infection,the susceptible DBA/2 mice and resistant BALB/c mice were infected by intraperitoneal injection of 1 106 P.c.chabaudi AS parasitized erythrocytes.Parasitemia of individual mice was monitored by microscopic examination of Giemsa-stained blood smear.Spleen cell suspension was prepared from the mice sacrificed on days 0,3,5,8 post infection(p.i.),while DCs were purified by magnetic separation and cultured in vitro.The levels of IL-12p40,IL-10 and TGF-(l in DCs supernatants were dynamically detected by ELISA.The level of IL-12p40 in DCs supernatants showed obvious increases on day 3-8 p.i.in DBA/2 mice,while significantly elevated on day 5-8 p.i.in BALB/c mice.Moreover,the level of IL-12p40 in BALB/c mice was significantly lower than that in DBA/2 mice from day 3 to 8 p.i..Levels of IL-10 and TGF-(l in DCs supernatants showed obvious increases on day 5-8 p.i.in BALB/c and DBA/2 mice.But the levels for DBA/2 mice were lower than those of BALB/c mice.These results indicated that there are significant differences in cytokine secretion profile of DCs in BALB/c and DBA/2 mice during the early stages of P.c.chabaudi AS infection.
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2010, Vol. 26 
