Abstract:Samples from two suspected anthrax outbreaks caused by slaughtering infected cattle,died from illness,in pasturing area of Qinghai Province in 2008 were isolated to detected Bacillus anthracis.The collected samples from patients,animals and environment were isolated and cultured.And Bacillus anthracis were identified by biochemistry experiment,phage-based splitting assay and penicillin test.Bacillus anthracis were detected from all animal samples,but none in the patients and environment samples.It's concluded that the detection rate of Bacillus anthracis is not only related to the level of detection technology but also to the quality and timeliness of sample collection.