Cloning,sequence analysis and linear B-cell epitopes prediction of full-length gene in coding region of lactate dehydrogenase from Plasmodium vivax Anhui
Abstract:The aim of this study is to clone and sequence the full-length gene in coding region of lactate dehydrogenase(LDH)from Plasmodium vivax(P.vivax)Anhui,and predict linear B-cell epitopes of P.vivax LDH(PvLDH)for the sake of providing a foundation on the development of rapid diagnosis method for vivax malaria based on P.vivax specifically monoclonal antibody.The PvLDH encoding gene from RNA of P.vivax Anhui was amplified by RT-PCR,and then the 951bp PCR-amplified product was cloned into pMD18-T vector and sequenced.The DNA and its protein sequence were analyzed and the linear B-cell epitopes of PvLDH were predicted.Sequence analysis for the cloned genes in pMD18-T vector showed one base in the full-length gene in coding region of LDH from P.vivax Anhui was different from that of P.vivax Sal-I and Belem,but there was no difference in deduced amino acid of PvLDH.A total of 12 linear B-cell epitopes of PvLDH were predicted from deduced protein sequence on line and a specific linear B-cell epitope was predicted by comparing with the linear B-cell epitopes of PfLDH.Results indicate that the PvLDH full-length gene in coding region is successfully cloned and a specific linear B-cell epitope is predicted successfully.
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