Abstract:The purpose of this study was to explore the method of isolation and identification of animal rotavirus(RV),and establish a fluorescence quantitative PCR(FQ-PCR)method for porcine RV(PRV).RV was separated with sensitive MA-104 cells and VP6 gene nucleotide of the separated RV strain was sequenced.The homology was also compared with the reference strains(AV-55)of PRV.FQ-PCR method was established for detection of PRV.Results showed that the cytopathic effects(CPE)were observed after 18 hours of inoculation.One RV strain was isolated and identified as PRV by VP6 nucleotide sequencing and its homology was 88.50% with AV-55.It's suggested that the established FQ-PCR detection method for PRV has higher sensitivity and greater specificity than common PCR or ELISA methods.
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