Abstract:The aim of this study was to improve prokaryotic expression level of glycoprotein (GP) gene in rabies virus. With the templet of full-length gene plasmid of rabies virus HEP-Flury strains,the gene without signal peptides (G1) and the extra-membrane gene (G2) were obtained by PCR respectively. The codons of glycoprotein gene's antigen epitope were modified and the enrich epitope gene was synthesized in order to get the gene G3 and match with the expression system of prokaryotic cell. The gene G1,G2 and G3 were sub-cloned into prokaryotic expression plasmid pET32a (+) respectively. After these three recombinant plasmids were transformed to BL21,the bacteria were induced by IPTG. The expression proteins were detected with SDS-PAGE,Western -blotting test and thin layer scanning. Results showed that the expression level of gene G3 was notably higher than that of gene G1 and gene G2. And the expression of glycoprotein gene in E. coli could be successfully improved by codon modification.
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