Abstract:In order to explore the natural apoptosis in the protoscolex of hydatid cyst,protoscoleces of hydatid cyst from sheep livers or lungs were collected and the morphology was examined directly under microscope. Protein expressions of caspase-1 and caspase-3 were detected by SP immunohistochemical technique. The apoptosis were examined by Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay (TUNEL) and the ultrastructural changes were observed by transmission electron microscope (TEM). Results showed that some withered and dim protoscoleces were found under microscope in natural protoscolex as well as the strong positive expressions of caspase-1 and caspase-3 were found in them. TUNEL assay has detected intensive apoptotic cells and TEM has observed typical ultrastructural changes of apoptosis. It demonstrated that protoscolex of hydatid cyst has apoptotic phenomenon naturally.
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