Abstract:In order to investigate a patient who was suspect to be infected with Human Granulocytic Anaplasmosis (HGA) in Shandong Province, epidemiological investigations were carried out in this case according to Epidemiological Investigation Scheme on HGA and blood samples were collected for laboratory tests. Nested-PCR assay was used to detect the 16S rRNA gene of HGA in blood samples and sequence analysis was followed. The patient showed typical clinical symptoms and epidemiological characterizations of HGA. PCR amplification result was positive and sequence analysis demonstrated that the homology of nucleotide sequences was 99.7% comparing with the corresponding parts of HGA found in wild mice in Zhejiang and Jilin Province. According to the registered GenBank sequences, the detected 16S rRNA gene was different at only one bps which lied in the 117 bps. This case was one of the cases reported during the first outbreak of public health incident about HGA in 2008, and was confirmed by laboratory tests and diagnosed as HGA according to the criteria in the guide book of HGA control and prevention issued by Ministry of Public Health. This report indicated that the HGA infection in human has existed in Shandong Province and it’s necessary to carry out further epidemiological investigations in the natural foci.
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