Abstract：The objective of the present study was to construct the full-length cDNA plasmid library for adult worms of Taenia saginata and then to form a gene expression pattern in order to establish a foundation for effective vaccine candidate antigen research and for comparative study of three main kinds of human Taenia. The mRNA was extracted from the T. saginata adult worms and the cDNA was synthesized. Products were digested by proteinase K and SfiⅠ, and then fractionated by CHROMA SPIN -400 column. The fractions were characterized by 1.1% agarose/EB gel electrophoresis, and the full-length cDNA plasmid library pBluescript II SK was constructed. The titer of the amplified library was evaluated and the quality of the constructed library was tested by PCR amplification using amphi-primers of the vector cloning sites.Furthermore,the positive recombinant clones were carried out with the 5’ terminus sequencing in large scale and then merged to unigenes. The results indicated that the titer of amplified library was 1016 pfu/μL. Of 1023 clone sequences were obtained and in with 419cDNA were merged. It was evident that the high-quality full-length cDNA plasmid library of adult T. saginata has been constructed successfully.
〔1〕蒋次鹏.牛带绦虫病的国内流行和中医药治疗〔J〕.地方病通报,2001,16(1):103-104.
〔2〕李文,马文祥.带绦虫病在我国流行现状〔J〕.河南预防医学杂志,1995,6(1):109-111.
〔3〕黄江,胡旭初,包怀恩,等.亚洲带绦虫成虫全长cDNA质粒文库的构建及EST测序〔J〕.热带医学杂志,2007,7(2):116-118.
〔4〕黄江,胡旭初,徐劲,等.猪带绦虫成虫cDNA质粒文库的构建及EST测序〔J〕.中国人兽共患病杂志,2008,24(12):1126-1128.
〔5〕陈守义,余新炳.华支睾吸虫囊蚴cDNA表达文库的构建及鉴定〔J〕.热带医学杂志,2003,3(1);22-27.
〔6〕吴军,裴福全,钟肖芬.华支睾吸虫cDNA文库的构建〔J〕.中国人兽共患病杂志,2003,19(4)91-92.
〔7〕吴忠道,余新炳,徐劲.日本血吸虫(大陆株)成虫基因表达谱的研究〔J〕.中山医科大学学报,2002,23(6)401-404.
〔8〕公茂庆,高永娥,赵中平.猪带绦虫病和囊虫病研究进展〔J〕.中国寄生虫病防治杂志,1996,9(2):l42-143.
〔9〕Flisser A,Gauci CG,et al.Induction of protection against porcine cysticercosis by vaccination with recombinant oncosphere anti-gens〔J〕.Infect I mmun,2004,72(9):5292-7.
〔10〕Flisser A,Sarti E,Lightowlers M,et al.Neurocysticercosis:re-gional status,epidemiology,i mpact and control measures inthe Americas〔J〕.Acta Trop,2003,87(1):43-51.
〔11〕Ito A,Wandra T,Yamasaki H,Cysticercosis/taeniasis in Asia and the Pacific〔J〕.Vector Borne Zoonotic Dis,2004,4(2):95-107.
