Abstract:To compare and analyze the immunoreactivity difference between Plasmodium vivax antigen and P.falciparum antigen by using immunoblotting with P.v patient's sera and pan-species specific Monoclonal antibody M26-32,and to find potentially diagnostic antigen for malaria.Plasmodipur filtration and 60% Percoll density gradient centrifugation techniques were used to purify parasite infected RBC from patient blood.Soluble antigen of P.f and P.v were prepared followed by immunoblotting analysis with P.v patient's sera and M26-32 McAb as primary antibody.Immunoblotting results showed that pool P.v patient's sera could specifically recognize 26,33,49,115 kDa P.v antigen band,100,102,110,150,175 kDa P.f antigen band,and cross-recognized 31,59,63,70,120kDa of both P.v and P.f antigen band.It is concluding that pool P.v patient's sera can specifically recognize P.v antigen and cross-recognize P.f antigen.Furthermore,31 kDa antigen has strong immunogenity and can be recognized by M26-32 McAb,the potential diagnostic value to human malaria need further study.
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