Abstract:To analyze the full length sequence of swine strain of hepatitis E virus(HEV)swChinash,11 overlapping fragments of swChinash genomes were amplified with reverse transcription nested polymerase chain reaction(RT-nPCR)and the 5'and 3'ends were amplified with RACE.The PCR products were cloned into Pmd18-T vector and sequenced.The result showed that its genome consisted of 7 235nt excluding the ploy(A)tail,and contained three ORFs(ORF1-3)that encoded proteins of 1707,660,122aa.sequence analysis revealed that the overall nucleotide sequence identity of swChinash was 73.3%-76.3% and 83.4%-84.7% with HEV genotype Ⅰ-Ⅲ and Ⅳ,respectively.It shared 84.7% nucletotide identity with China 8 human strain.Necletotide identity analysis based on three ORFs and the full length sequence yielded similar results:it belongs to genotype Ⅳ.However,phylogenetic analyses suggests that it represents a distinct variant among the genotype Ⅳ isolates.
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