Abstract:To observe the changes of 10 cytokines in serum of patients with hemorrhagic fever with renal syndrome(HFRS) and to evaluate its mechanism of action,53 cases of patients with HFRS were prospectively selected for study,including 9 mild cases,16 moderate cases,15 severe cases and 13 dangerous cases.Serum samples of patients were collected at different stages of disease,both before and after treatment with hematodialysis.Meanwhile.20 healthy adults were selected as normal controls.The serum levels of cytokines(IL-1β,IL-2,IL-4,IL-6.IL-8.IL-10,IL-12,IL-15,IFN-αand TNF-αwere detected by ELISA assay and the results were analyzed according to 3 items;stage and pattern of disease as well as the effect of intervention with hematodialysis.Compared with the normal controls,the serum levels of these cytokines tested were significantly elevated in HFRS patients during febrile stage with peak values in the hypotensive-oligouric stage(P<0.01).It is evident that these 10 kinds of cytokines may be associated with the development of inflammation in HFRS during febrile stage,in which the pro-inflammatory and anti-inflammatory cytokines may be under an unbalanced status.The over secretion of cytokines TNF-α,IL-1β,IL-2.IL-4,IL-6,IL-8,IL-10,IL-12 and IL-15 may lead to the development of systemic inflammatory reactions,whereas the insufficient secretion of IFN-α may limit the function of cell-mediated immunity which is unsuitable for the control of inflammation.As to the effect of hematodialysis,it seems to decrease the amounts of cytokines secreted,thus lessening the damages induced by inflammation.
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