Abstract:According to the P32 gene sequence of capriopox virus(CPV)and H2L gene sequence of orf virus(ORFV),two pairs of primers special for CPV and ORFV were designed and synthesized respectively,and then a rapid duplex PCR assay for differentiating CPV and ORFV was developed.A 969bp fragment special for CPV and a 507bp fragment for ORFV were amplified,and no product was amplified from the skin samples of normal healthy goat and sheep or the other pathogens suckas vaccnina virus and foot and mouth virus.The sensitivity test of duplex PCR showed that 10 1.66TCID_ 50 goat poxvirus(GPV)and 10 2.33 TCID_ 50 ORFV could be detected,respectively.A total of 11 field clinical skin tissue samples collected form Gansu province were analyzed by duplex PCR,the results showed that 8 samples were positive in CPV and 3 in ORFV respectively,which were identical with the result of virus isolation and identification.
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