Abstract：In the present study,a RT-nested-PCR assay was established for the detection of rabies virus in which two set of primers were designed from the nucleotide sequence of N gene,and the condition of RT-nested-PCR assay was validated. It was found that this assay could be used for the specific and stable detection of rabies virus from dog's saliva with a limit of detection of 11.2 fg RNA. It is concluded that a sensitive and rapid method for the detection of rabies virus from dog's saliva was successfully established,which provide a new means for further studies on the rapid diagnosis for rabies virus.
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