Abstract：To prepare and identify the monoclonal antibody against Borrelia burgdorferi in order to provide basis for the detection of Lyme spirochetes and diagnosis of Lyme disease.Spleen cells were prepared from BALB/c mice which was immunized by the whole proteins of B.garinii strain PD91.And then they were fused with SP2/0 myeloma cells.The positive clones were picked out and identified by ELISA and WB.The positive clones were subcloned two or three times to obtain the monoclonal cells.Ten hybridoma cell strains which could produce the monoclonal antibody against B.burgdorferi were obtained,in which four is against OspA,three against OspB and three against OspC.Three kinds of monoclonal antibodies against B.burgdorferi have been successfully prepared.And they can be used in the diagnosis of Lyme disease and detection of Lyme spirochetes.
[1]Tom G Schwan,Willy Burgdorfer,Patricia A Rosa.Borrelia[J].Manual of Clinical Microbiology,2003,1:746-758.
[2]张哲夫,万康林,张金声,等.我国莱姆病的流行病学和病原学研究[J].中华流行病学杂志,1997,18:8-11.
[3]Coleman J L,R C Rogers,J L Benach.Selection of an escape va-riant ofBorrelia burgdorferiby use of bactericidal monoclonal antibodies to OspB[J].Infect Immun,1992,60:3098-3104.
[4]Kramer MD,Schaible UE,Wallich R,et al.Characterization of Borreliaburgdorferiassociated antigens by monoclonal antibod-ies[J].Immunobiology,1990,181:357-366.
[5]徐志凯.实用单克隆抗体技术[M].西安:陕西科学技术出版社,1992:27-36.
[6]Timothy J Sullivan,Karim E Hechemy,Hervie L Harris,et al.Monoclonal antibody to native P39Protein fromBorrelia burg-dorferi[J].J Clin Microbiol,1994,32(2):423-429.
[7]Barbour A.,Tessier SL,Hayes SF.Variationin a major surface protein of Lyme disease spirochetes[J].Infect Immun,1984,45:94-100.
[8]Bruckbauer HR,Preac-Mursic V,Fuchs R,et al.Cross-reactive proteins ofBorreliaburgdorferi[J].Eur J Clin Microbiol Infect Dis,1992,11:224-232.
[9]Jonsson M,Noppa L,Barbour AG,et al.Heterogeneity of out-er membrane proteins inBorrelia burgdorferi:comparison of ospoperons of three isolates of different geographic origins[J].Infect Immun,1992,60:1845-1853.
[10]Berg D,Abson KG,Prose NS.The laboratory diagnosis of Lyme disease[J].Arch.Dermatol,1991,127:866-870.
