Abstract：To characterize growth kinetics model of different subtypes of Vibrio parahaemolyticus and to provide useful data for developing risk assessment of V. parahaemolyticus in seafood, PCR and RAPD techniques were applied to molecular characterization of 5 strains of V. parahaemolyticus isolated from different sources and the growth of bacterial at 37℃ and 15℃ were analyzed. Growth curves were fitted using modified Gompertz equation and kinetic parameters of different strains and predictive model were developed with nonlinear estimation equations. The results showed that 4 strains of V. parahaemolyticus from food poisonous accident samples had genotype of tl+tdh+trh-toxR+, however, one isolate from sea products was tl+tdh-trh-toxR+. An efficient random primer named P21 was selected to divide these strains into five subtypes. Growth curves at 37℃ had no significant difference among the five subtypes of strains, but when grown at 15℃, generation time and lag time of 5 strains ranged from 0.727h to 1.062h and 10.467h to 12.552 h, respectively, suggesting that different V. parahaemolyticus strains had different cold adaptive ability. Predicitive model based on growth kinetic parameters of VP05166 was developed and the predicted value could agree with observed value well at 37℃(RMSE<0.5), but difference between predicted value and observed value of VP06003 and VP06127 at 15℃ were significant (RMSE>1). These results implicated that information on growth characters variation of V. parahaemolyticus strains were needed to improve reliability of risk assessment of V. parahaemolyticus in sea products.
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