Abstract:With the genome of AIV H5N1 subtype in Genbank,we had designed 8 pairs of primers,cloning the 8 fragments from the A/Duck/Guangdong/DG01/05(H5N1 subtype,abbre:DG01 strain),sequenced and analyzed.The sequences indicated that the genome of DG01 strain was composed of 8 fragments,which were PB2 2 341bp,PB1 2 341bp,PA 2 233bp,HA 1 779bp,NP 1 565bp,NA 1 398 bp,M 1 027bp,NS 875 bp,coding for 11 proteins.Compared with the most stainsin Mainland strains,The DG01 strain shared the amino acid sequence as RERRRKKR at the cleavage site between HA1 and HA2,which was the feature of the highly pathogenic avian influenza virus.DG01 had amino acids deletion in the NA stalk from the sites 49 to 68 and NS1 from 80 to 84,which were prominet characteristics of the H5N1 strains isolated from mainland China in recent years.
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