Abstract：To find a suitable method of isolation and cultivation in vitro for the Trichinella nelsoni muscle larval cells,muscle larval were first isolated by digestion method,and then separated through homo-genization. The morphology of the isolated cells and their diameter of cells and nuclei were observed and measured under inverted phase-contrast microoscopy.In addition, the cells were cultivated in RPMI-1640 medium containing 10% fetal calf serum and then identified by multiplex PCR. The results showed that the average diameters of the separated cells from T.nelsoni muscle larvae and their nuclei were 11.72±1.13 μm and 5.76±0.68 μm respectively. The cells began to adhere to the substratum of culture flask 24-72 hours after they were inoculated into medium and the adhered cells on the flask appeared to be bright,turgid and round after 8-12 days cultivation. As detected by the multiplex PCR assay,the same bands could be demonstrated between the cultured cells and the T.nelson muscle larval cells.The T.nelson muscle larval cells could survive at least for 20 days in RPMI-1840 medium containing 10% fetal calf serum.
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