Abstract：The genomic DNA of Leptospira interrogans serovar Lai strain Lai was used as template to amplify gene LA0202 by PCR and the amplified product was then linked to prokaryotic expression vector pET28b(+). The recombinant plasmid was digested with BamHI and HindIII and then transformed to E.coli BL21 cells to express the recombinant protein. The recombinant protein LA0202 was identified with SDS-PAGE and the hemolytic activity of this protein was determined using sheep blood agar plate method. RT-PCR assay was used to detect the transcription of LA0202 gene by culturing L.interrogans strain Lai in vitro. It was demonstrated that the recombinant protein LA0202 was successfully expressed in E.coli BL21 cells and its hemolytic activity could be confirmed using the sheep blood agar plate method. In addition, the transcription of the LA0202 gene was identified in vitro by RT-PCR assay. It is evident that LA0202 gene transcribed in vitro, and it may be the novel hemolytic gene in the genome of L.interrogans.
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