Abstract：To study whether the vaccinia virus Tian Tan strain(VTT) can infect equine cells and produce immune response in horses,recombinant vaccinia virus WR strain expressing green fluorescence protein(GFP) was used to infect fetal dermal donkey cells(FDD) and the virus replication was tested regularly by flow cytometry.The optional infection time was observed.HIV-1 Gag protein-expressing recombinant VTT(rVTT) was further used to infect FDD cells.Infection was confirmed by western blotting and intracellular protein staining.Finally,horses were vaccinated with VTT to inspect the immune response induced.Both strains of vaccinia virus could infect FDD cells and inducs cytopathic effects.Optimal infection time of FDD cells was 48h,at which point,binding antibodies could be detected in the horses sera.These results study suggest that the vector vaccine with recombinant eqaine infcetion anemia virus(EIAT) antigen of vaccinaa virus may be useful for vaccination to horses.
