Abstract:Streptococcus equi subsp.zooepidemicus can cause swine diseases,thus making substantial economic losses in recent years.To evaluate the antigenic variability among 10 strains of Streptococcus equi subsp,zooepidemicus of pig-origin isolated in China,the formalin-inactivated whole bacterial cells of these 10 local strain were immunized to inbred ICR mice of 12 weeks old with addition of the complete Freund's ajuvant(CFA),and re-immunized 4 weeks later with a similar dose of bacteria emulsified in imcomplete Freund's adjuvant.Two weeks after immunizations,the vaccinated and the control mice were all challenged intraperitoneally with 5 LD_(50) of homologeous strain and 1.6×10~(5) CFU of ATCC35246 strain.The result demonstrated that the immunization protection rate of mice challenged with the homologeous strain was above 90%,while those of mice challenged with ATCC35246 was around 80%-100%,with no significant difference.From these observations,it is evident that no great antigenic variability can be detected among these 10 local strains of S.equi subsp.zooepidemicus isolated in China,and this result would favor the use of single strain of bacteria for the preparation of vaccine.
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