Abstract:To analyse the allelic polymorphism in the merozoite surface protein 3α of Plasmodium vivax(PvMSP-3α),the nested PCR was used to amplify the genes encoding to PvMSP-3α and PvMSP-1,and the PCR-amplified product of PvMSP-3α was digested by Hha 1 restriction endonuclease and analyzed by restriction fragment length polymorphism(RFLP) studies.The experimental results showed that three kinds of genotypes of PvMSP-3α could be demonstrated in three different field isolates,in which the proportions of type I,II and III accounted for 77.42%,6.45% and 16.13% respectively.Of the 24 isolates from Yunnan province,the percentages of type I,II and III were 79.17%,4.17% and 16.17% respectively.All the type II and III of PvMSP-3α were matched with the Belem type of PvMSP-1,while the Sal-1 type of PvMSP-1 belonged to the type I of PvMSP-3α.It is concluded that the PvMSP-3α from field isolates of Yunnan province is highly diverse,and the relationship of PvMSP-3α with PvMSP-1 remains to be studied furthermore.
[1]Galinski MR,Corredor-Medina C,Povoa M,et al.Plasmodiumvivaxmerozoite surface protein-3 contains coiled-coil motifs in analanine-rich central domain〔J〕.Mol Biochem Parasitol,1999,101(1-2):131-47.
[2]Han ET,Song TE,Park JH,et al.Allelic dimorphism in themerozoite surface protein-3alpha in Korean isolates ofPlasmodi-um vivax〔J〕.Am J Trop Med Hyg,2004,71(6):745.
[3]Foley M,Ranford-Cartwright LC,Babiker HA.Rapid and sim-ple method for isolating malaria DNA from fingerprick samples ofblood〔J〕,Mol Biochem Parasitol,1992,70:241-244.
[4]Bruce MC,Galinski MR,Barnwell JW,et al.Polymorphism atthe merozoite surface protein-3alpha locus ofPlasmodium vivax:global and local diversity〔J〕.Am J Trop Med Hyg,1999,61(4):518.
[5]Bruce MC,Galinski MR,Barnwell JW,et al.Genetic diversityand dynamics of Plasmodium falciparum andPlasmodium vivaxpopulations in multiply infected children with asymptomatic ma-laria infections in Papua New Guinea〔J〕.Parasitology,2000,121(Pt 3):257.
[6]Cui L,Mascorro CN,Fan Q,et al.Genetic diversity and multi-ple infections ofPlasmodium vivaxmalaria in Western Thailand〔J〕.Am J Trop Med Hyg,2003,68(5):613.
[7]Kolakovich KA,Ssengoba A,Wojcik K,et al.Plasmodiumvivax:favored gene frequencies of the merozoite surface protein-1 and the multiplicity of infection in a malaria endemic region〔J〕.Exp Parasitol,1996,83(1):11.
[8]张山鹰,许龙善,陆惠民.间日疟原虫裂殖子表面蛋白的等位基因型检测〔J〕.中国寄生虫学与寄生虫病杂志,2004,22(2):86-89.
[9]张山鹰,许龙善,黄天谊,等.间日疟原虫MSP-1和CSP基因遗传多样性的分析〔J〕.中国寄生虫病防治杂志,2005,18(2):104-108.