Effect of mouse embryonic fibroblast feeder layer and liver matrix of rabbit on the activities of LDH and ACP in cultured cells from schistosomula Schistosoma japonicum
Abstract：To study the effect of feeder layer with mouse embryonic fibroblast (MEF) or/and with liver matrix of rabbit on the activities of LDH, ACP in the cultured cells from schistosomula Schistosoma japonicum, the cells from 21 day-old schistosomula were inoculated on small glass coverslips with or without liver matrix smeared previously. Cells with matrix were divided into two groups respectively: one of which was cultured in routine medium that RPMI-1640 containing 20 % calf serum with a moderate amount of antibiotics, the other was cultured in routine medium containing MEF feeder layer. Cells inoculated without liver matrix were also divided into two groups which were cultured in routine medium and MEF co-culture system respectively. So there were four groups in our experiments: liver matrix group, co-culture group, control group and MEF group. The cells were stained to show the activities of LDH and ACP after cultured for 7 days. It was found that the activities of LDH and ACP in the cultured cells were strongest in the cells of co-culture group, then in the MEF group and the liver matrix group, the activities were weakest in the control group. Quantitative analysis showed that activity of LDH had significant difference (P<0.01) among those four groups. The activity of ACP was higher in the co-culture group and the MEF group than the control group (P<0.01); there was no significant difference between the liver matrix group and the control group (P>0.05); but the co-culture group , the MEF group and the liver matrix group were statistically different (P<0.01). It was suggested that the MEF feeder layer can promote metabolism of cultured cells from schistosomula. Further more, the MEF and the liver matrix have a co-effect on the activity of cells from schistosomula.
