Abstract：To confirm the five clinically suspected cases of dog rabies isolated in Chongqing emerging from December of 2004 to April of 2005 by conventional laboratory techniques and virus isolation,the brain tissue samples were subjected to the detection of nucleoprotein gene of rabies virus by RT-PCR,then followed by viral isolation and characterization using the mice inoculation test(MIT) and the direct fluorescent antibody test(FAT).Afterward nucleotide sequence comparison and phylogenetic analysis of the isolated virus strains were conducted.The brain tissues of these five suspected dog rabies cases showed positive amplification of N gene in RT-PCR and MIT resulted in the isolation of corresponding 5 rabies virus strains,named as CQ/ws-1,CQ/wx-1,CQ/wl-1,CQ/fj-1 and CQ/qj-1,respectively upon the places where the cases appeared.The sequence comparison and phylogenetic analysis by using 3' part of N gene showed that all 5 strains belong to genotype I Lyssavirus and are the classical rabies viruses, but 5 strains also showed some sequence diversities,indicating they were not identical and likely have different epidemiological origin.The five suspected dog cases emerging in five counties of Chongqing in 2004/2005 were confirmed as rabies and the viruses isolated were of genotype Ⅰ based on phylogenetic analysis.The results showed that animal rabies in Chongqing probably has different epidemiological origins and is predominated by genotype Ⅰ rabies viruses.
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